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Generation of a rabbit VH domain antibody polyspecific to c-Met and adenoviral knob protein

Authors
 Shin-Young Im  ;  Ki Su Kim  ;  Chae-Ok Yun  ;  Joo-Hang Kim  ;  Kye-Sook Yi  ;  Junho Chung 
Citation
 Biochemical and Biophysical Research Communications, Vol.339(1) : 305-312, 2006 
Journal Title
 Biochemical and Biophysical Research Communications 
ISSN
 0006-291X 
Issue Date
2006
Abstract
Several types of bispecific antibodies with affinity to both adenoviral coat proteins and a targeted antigen have been developed with the aim of providing the specific delivery of adenoviral gene therapy vehicle. From a phage display library of combinatorial dAb2s (each with an anti-adenoviral knob protein VH fragment linked with an anti-c-Met VH), we serendipitously enriched and isolated a clone, JS5, that has polyspecificity such that it binds both the adenoviral knob protein and c-Met, despite having only one VH domain. Our indirect observations suggest that the polyspecificity of JS5 is developed through accumulation of antibody specificity. The method of sequential immunization of a rabbit, first with the adenoviral knob protein and then with target antigens, may provide a method by which monoclonal antibodies with stand-alone polyspecificity may be developed. Such targeted polyspecific antibodies could readily be used for re-directing adenoviral vectors to target cells.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X05025192
DOI
10.1016/j.bbrc.2005.10.208
Appears in Collections:
5. Research Institutes (연구소) > Institute for Cancer Research (암연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
김주항(Kim, Joo Hang)
윤채옥(Yun, Chae Ok)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110517
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