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Generation of a rabbit VH domain antibody polyspecific to c-Met and adenoviral knob protein

DC FieldValueLanguage
dc.contributor.author김주항-
dc.contributor.author윤채옥-
dc.date.accessioned2015-06-10T12:49:40Z-
dc.date.available2015-06-10T12:49:40Z-
dc.date.issued2006-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/110517-
dc.description.abstractSeveral types of bispecific antibodies with affinity to both adenoviral coat proteins and a targeted antigen have been developed with the aim of providing the specific delivery of adenoviral gene therapy vehicle. From a phage display library of combinatorial dAb2s (each with an anti-adenoviral knob protein VH fragment linked with an anti-c-Met VH), we serendipitously enriched and isolated a clone, JS5, that has polyspecificity such that it binds both the adenoviral knob protein and c-Met, despite having only one VH domain. Our indirect observations suggest that the polyspecificity of JS5 is developed through accumulation of antibody specificity. The method of sequential immunization of a rabbit, first with the adenoviral knob protein and then with target antigens, may provide a method by which monoclonal antibodies with stand-alone polyspecificity may be developed. Such targeted polyspecific antibodies could readily be used for re-directing adenoviral vectors to target cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent305~312-
dc.relation.isPartOfBiochemical and Biophysical Research Communications-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleGeneration of a rabbit VH domain antibody polyspecific to c-Met and adenoviral knob protein-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentInstitute for Cancer Research (암연구소)-
dc.contributor.googleauthorShin-Young Im-
dc.contributor.googleauthorKi Su Kim-
dc.contributor.googleauthorChae-Ok Yun-
dc.contributor.googleauthorJoo-Hang Kim-
dc.contributor.googleauthorKye-Sook Yi-
dc.contributor.googleauthorJunho Chung-
dc.identifier.doi10.1016/j.bbrc.2005.10.208-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00945-
dc.contributor.localIdA02614-
dc.relation.journalcodeJ00281-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X05025192-
dc.contributor.alternativeNameKim, Joo Hang-
dc.contributor.alternativeNameYun, Chae Ok-
dc.contributor.affiliatedAuthorKim, Joo Hang-
dc.contributor.affiliatedAuthorYun, Chae Ok-
dc.rights.accessRightsnot free-
dc.citation.volume339-
dc.citation.number1-
dc.citation.startPage305-
dc.citation.endPage312-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, Vol.339(1) : 305-312, 2006-
Appears in Collections:
5. Research Institutes (연구소) > Institute for Cancer Research (암연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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