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Multiplex Latex Bead 유세포 분석기 기법과 Quellung 반응을 이용한 폐구균 혈청형 분석 비교

Other Titles
 Comparison of Multiplex Latex Bead Flow Cytometric Analysis and Quellung Analysis in Serotyping Pneumococci 
Authors
 최경민  ;  연수인  ;  김은숙  ;  신전수  ;  용동은  ;  이경원  ;  김동수 
Citation
 Korean Journal of Pediatric Infectious Diseases (소아감염), Vol.13(2) : 99-105, 2006 
Journal Title
 Korean Journal of Pediatric Infectious Diseases (소아감염) 
ISSN
 1226-3923 
Issue Date
2006
Keywords
Pneumococci ; Serotype ; Quellung reaction ; Multiplex latex bead flow cytometry analysis
Abstract
Purpose : The serotyping results of the Quellung reaction was compared with the newly developed multiplex latex assay and the sensitivity of the Quellung reaction was compared. Methods : We checked the serotypes of 35 samples obtained from patients treated at Yonsei University Medical Center using the multiplex latex bead method and compared the results with the serotypes previously obtained via the Quellung reaction. Results : A decrease in the mean fluorescence was detected in the samples tested with the multiplex assay. Seventeen samples out of the 27 samples agreed to the results of the Quellung assay. We were only able to confirm the concordance of 11 serotypes out of 14 serotypes available. Conclusion : The Quellung reaction is time consuming procedure and prone to errors even with expertise in the procedure, and other alternate methods in serotyping have been investigated to overcome these problems. The newly developed multiplex latex bead assay can test more samples at the same time and has a higher degree of sensitivity. A large scale trial is required to test the sensitivity of the new assay across various serotypes along with efforts to increase the sensitivity of the Quellung assay. The preliminary data suggests that this method may be widely used.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Soo(김동수)
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110277
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