Hydrogen peroxide upregulates TNF-related apoptosis-inducing ligand (TRAIL) expression in human astroglial cells, and augments apoptosis of T cells

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MeSH: Allergy and Immunology ; Apoptosis* ; Astrocytes/metabolism* ; Cell Line, Tumor ; Cyclosporine/pharmacology ; Gene Expression Regulation, Neoplastic* ; Humans ; Hydrogen Peroxide/pharmacology* ; Hypoxia ; Immunosuppressive Agents/pharmacology ; Oxidative Stress ; Ribonucleases/metabolism ; T-Lymphocytes/metabolism* ; TNF-Related Apoptosis-Inducing Ligand/biosynthesis* ; Up-Regulation*

Keywords: Astroglial cells ; hydrogen peroxide ; TRAIL ; cyclosporin A ; apoptosis ; oxidative stress ; neuroimmunology

Abstract: The brain is particularly vulnerable to oxygen free radicals, and these radicals have been implicated in the pathology of several neurological disorders. In this study, the modulation of TNF-related apoptosis-inducing ligand (TRAIL) expression by oxidative stress was shown in LN215 cells, an astroglioma cell line. Hydrogen peroxide (H2O2) treatment increased TRAIL expression in LN215 cells and H2O2-induced TRAIL augmented apoptosis in Peer cells, a cell line sensitive to TRAIL-mediated cell death. Our findings suggest that the upregulation of TRAIL in astroglial cells may abrogate immune cell effector functions.