Identification of DNA copy-number aberrations by array-comparative genomic hybridization in patients with schizophrenia
Authors
Ho Jin Moon ; Sung-Vin Yim ; Woon Kyu Lee ; Yang-Whan Jeon ; Young Hoon Kim ; Young Jin Ko ; Kwang-Soo Lee ; Kweon-Haeng Lee ; Sang-Ick Han ; Hyoung Kyun Rha
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.344(2) : 531-539, 2006
Chromosomal abnormalities are implicated as important markers for the pathogenesis in patients with schizophrenia. In this study, with using bacterial artificial chromosome (BAC) array-based comparative genomic hybridization (CGH), we analyzed DNA copy-number changes among 30 patients with schizophrenia. The most frequent changes were partial gain of Xq23 (52%) and loss of 3q13.12 (32%). Other frequent gains were found in: 1p, 6q, 10p, 11p, 11q, 14p, and 15q regions, and frequent losses were found in: 2p, 9q, 10q, 14q, 20q, and 22q regions. The set of abnormal regions was confirmed by real-time PCR (9q12, 9q34.2, 11p15.4, 14q32.33, 15q15.1, 22q11.21, and Xq23). All real-time PCR results were consistent with the array-CGH results. Therefore, it is suggested that array-CGH and real-time PCR analysis could be used as powerful tools in screening for schizophrenia-related genes. Our results might be useful for further exploration of candidate genomic regions in the pathogenesis of schizophrenia.