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Kainic acid 유발 마우스 간질모델에서 minocycline에 의한 caspase 의존성 세포고사 억제

Other Titles
 Minocycline Ingibits Caspase-Dependent Cell Death Pathway and is Neuroprotective against Hippocampal Damage after Kainic Acid-Induced Seizure in Mice 
 신하영  ;  조양제  ;  조경주  ;  김현우  ;  김현정  ;  김경환  ;  이병인  ;  허경 
 Journal of Korean Epilepsy Society (대한간질학회지), Vol.10(1) : 3-10, 2006 
Journal Title
Journal of Korean Epilepsy Society(대한간질학회지)
Issue Date
Minocycline ; Seizure ; Apoptosis ; Caspase ; Cytochrome c
Purpose: Despite current acceptance of its neuroprotective property, whether the minocycline affords neuroprotection or how it protects neurons against seizures in the animal model of epilepsy is not clear. This prompts us to investigate whether minocycline is neuroprotective against kainic acid (KA)-induced seizure in mice through inhibition of caspase-dependent mitochondrial apoptotic pathways.
Methods: Adult male ICR mice were subjected to seizures by intrahippocampal KA injection with treatment of vehicle or minocycline. For cell death analysis, histological analysis using cresyl-violet staining, TdT-mediated dUTP-biotin nick end labeling (TUNEL), and histone-associated DNA fragmentation analysis were performed. Evaluation of cytochrome c, cleaved caspase-3, and caspase-3 activity were also performed.
Results: Hippocampal neuronal death was evident by cresyl violet staining, TUNEL, and cell death assay in vehicle-treated mice after KA injection; however, there was significant reduction of cell death in the minocycline-treated group. Significant decrease of both cytosolic translocation of cytochrome c and subsequent activation of caspase-3 after treatment of minocycline were demonstrated by Western blot analysis, immunohistochemical staining, and caspase-3 activity assay.
Conclusion: This study suggests that minocycline may be neuroprotective against hippocampal damage after KA-induced seizure through inhibition of caspase-dependent cell death pathways.
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1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Gyung Whan(김경환) ORCID logo https://orcid.org/0000-0001-7053-4372
Kim, Hyun Woo(김현우)
Kim, Hyun Jeong(김현정)
Lee, Byung In(이병인)
Cho, Kyuong Joo(조경주)
Cho, Yang Je(조양제)
Heo, Kyoung(허경)
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