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DA-9601, Artemisia Asiatica Herbal Extract, Ameliorates Airway Inflammation of Allergic Asthma in Mice

 Ji Young Kim  ;  Dae Yong Kim  ;  Yun Song Lee  ;  Bong Ki Lee  ;  Kyung-Hoon Lee  ;  Jai Youl Ro 
 MOLECULES AND CELLS, Vol.22(1) : 104-112, 2006 
Journal Title
Issue Date
Administration, Oral ; Animals ; Anti-Asthmatic Agents/administration & dosage ; Anti-Asthmatic Agents/pharmacology* ; Anti-Inflammatory Agents/administration & dosage ; Anti-Inflammatory Agents/pharmacology* ; Artemisia/chemistry* ; Asthma/chemically induced ; Asthma/drug therapy* ; Bronchoalveolar Lavage Fluid/cytology ; CD40 Antigens/biosynthesis ; CD40 Ligand/biosynthesis ; Cytokines/biosynthesis ; Extracellular Signal-Regulated MAP Kinases/biosynthesis ; Female ; Lung/metabolism ; Lung/pathology ; MAP Kinase Kinase 4/biosynthesis ; Mice ; Mice, Inbred BALB C ; NF-kappa B/biosynthesis ; Ovalbumin ; Plant Extracts/administration & dosage ; Plant Extracts/pharmacology* ; Vascular Cell Adhesion Molecule-1/biosynthesis
Allergic Asthma ; CD40 ; CD40L ; Goblet Cells ; IgE ; MAP Kinases ; Matrix Metalloproteinases (MMPs) ; NF-kB ; VCAM-1
We previously reported that DA-9601, ethanol herbal extract of Artemisia asiatica, inhibited histamine and leukotriene releases in guinea pig lung mast cells activated with specific antigen/antibody reaction. This study aimed to evaluate the inhibitory effect of DA-9601 on the OVA-induced airway inflammation in allergic asthma mouse model. BALB/c mice were sensitized and challenged with OVA. DA-9601 was administered orally 1 h before every local OVA-challenge. OVA-specific serum IgE was measured by ELISA, recruitment of inflammatory cells in BAL fluids and lung tissues by Diff-Quik and H&E staining, respectively, the expressions of CD40, CD40L and VCAM-1 by immunohistochemistry, goblet cell hyperplasia by PAS staining, activities of MMPs by gelatin zymography, expressions of mRNA and proteins of cytokines by RT-PCR and ELISA, activities of MAP kinases by western blot, and activity of NF-KappaB by EMSA. DA-9601 reduced IgE level, recruitment of inflammatory cells into the BAL fluid and lung tissues, expressions of CD40, CD40L and VCAM-1 molecules, goblet cell hyperplasia, MMPs activity, expressions of mRNA and productions of various cytokines, activities of MAP kinases and NK-KappaB increased from OVA-challenged mice. These data suggest that DA-9601 may be developed as a clinical therapeutic agent in allergic diseases due to suppressing the airway allergic inflammation via regulation of various cellular molecules expressed by MAP kinases/NF-KappaB pathway.
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Lee, Bong Ki(이봉기)
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