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In vivo determination of the gap2 gene promoter activity in Giardia lamblia

Authors
 Hye-won Yang  ;  Juri Kim  ;  Tai-soon Yong  ;  Soon-jung Park 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.44(1) : 21-26, 2006 
Journal Title
 KOREAN JOURNAL OF PARASITOLOGY 
ISSN
 0023-4001 
Issue Date
2006
MeSH
Animals ; Blotting, Southern/methods ; GTPase-Activating Proteins/genetics* ; Gene Expression/genetics ; Gene Expression/physiology* ; Gene Order ; Genes, Protozoan/genetics ; Genes, Protozoan/physiology* ; Genes, Reporter/genetics ; Genetic Engineering/methods ; Giardia lamblia/genetics* ; Life Cycle Stages/physiology ; Luciferases/genetics ; Luciferases/metabolism ; Plasmids ; Promoter Regions, Genetic/physiology* ; Recombinant Fusion Proteins/analysis ; Recombinant Fusion Proteins/biosynthesis ; Time Factors ; Transfection/methods
Keywords
Giardia lamblia ; gap2 gene expression ; encystation ; luciferase ; transfection
Abstract
A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.
Files in This Item:
T200600455.pdf Download
DOI
10.3347/kjp.2006.44.1.21
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109307
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