Cited 2 times in
In vivo determination of the gap2 gene promoter activity in Giardia lamblia
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박순정 | - |
dc.contributor.author | 용태순 | - |
dc.date.accessioned | 2015-06-10T12:09:57Z | - |
dc.date.available | 2015-06-10T12:09:57Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109307 | - |
dc.description.abstract | A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 21~26 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PARASITOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Blotting, Southern/methods | - |
dc.subject.MESH | GTPase-Activating Proteins/genetics* | - |
dc.subject.MESH | Gene Expression/genetics | - |
dc.subject.MESH | Gene Expression/physiology* | - |
dc.subject.MESH | Gene Order | - |
dc.subject.MESH | Genes, Protozoan/genetics | - |
dc.subject.MESH | Genes, Protozoan/physiology* | - |
dc.subject.MESH | Genes, Reporter/genetics | - |
dc.subject.MESH | Genetic Engineering/methods | - |
dc.subject.MESH | Giardia lamblia/genetics* | - |
dc.subject.MESH | Life Cycle Stages/physiology | - |
dc.subject.MESH | Luciferases/genetics | - |
dc.subject.MESH | Luciferases/metabolism | - |
dc.subject.MESH | Plasmids | - |
dc.subject.MESH | Promoter Regions, Genetic/physiology* | - |
dc.subject.MESH | Recombinant Fusion Proteins/analysis | - |
dc.subject.MESH | Recombinant Fusion Proteins/biosynthesis | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Transfection/methods | - |
dc.title | In vivo determination of the gap2 gene promoter activity in Giardia lamblia | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Hye-won Yang | - |
dc.contributor.googleauthor | Juri Kim | - |
dc.contributor.googleauthor | Tai-soon Yong | - |
dc.contributor.googleauthor | Soon-jung Park | - |
dc.identifier.doi | 10.3347/kjp.2006.44.1.21 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01545 | - |
dc.contributor.localId | A02424 | - |
dc.relation.journalcode | J01957 | - |
dc.identifier.eissn | 1738-0006 | - |
dc.identifier.pmid | 16514278 | - |
dc.subject.keyword | Giardia lamblia | - |
dc.subject.keyword | gap2 gene expression | - |
dc.subject.keyword | encystation | - |
dc.subject.keyword | luciferase | - |
dc.subject.keyword | transfection | - |
dc.contributor.alternativeName | Park, Soon Jung | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Park, Soon Jung | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 44 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 21 | - |
dc.citation.endPage | 26 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PARASITOLOGY, Vol.44(1) : 21-26, 2006 | - |
dc.identifier.rimsid | 52268 | - |
dc.type.rims | ART | - |
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