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In vivo determination of the gap2 gene promoter activity in Giardia lamblia

DC Field Value Language
dc.contributor.author박순정-
dc.contributor.author용태순-
dc.date.accessioned2015-06-10T12:09:57Z-
dc.date.available2015-06-10T12:09:57Z-
dc.date.issued2006-
dc.identifier.issn0023-4001-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/109307-
dc.description.abstractA shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.-
dc.description.statementOfResponsibilityopen-
dc.format.extent21~26-
dc.relation.isPartOfKOREAN JOURNAL OF PARASITOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBlotting, Southern/methods-
dc.subject.MESHGTPase-Activating Proteins/genetics*-
dc.subject.MESHGene Expression/genetics-
dc.subject.MESHGene Expression/physiology*-
dc.subject.MESHGene Order-
dc.subject.MESHGenes, Protozoan/genetics-
dc.subject.MESHGenes, Protozoan/physiology*-
dc.subject.MESHGenes, Reporter/genetics-
dc.subject.MESHGenetic Engineering/methods-
dc.subject.MESHGiardia lamblia/genetics*-
dc.subject.MESHLife Cycle Stages/physiology-
dc.subject.MESHLuciferases/genetics-
dc.subject.MESHLuciferases/metabolism-
dc.subject.MESHPlasmids-
dc.subject.MESHPromoter Regions, Genetic/physiology*-
dc.subject.MESHRecombinant Fusion Proteins/analysis-
dc.subject.MESHRecombinant Fusion Proteins/biosynthesis-
dc.subject.MESHTime Factors-
dc.subject.MESHTransfection/methods-
dc.titleIn vivo determination of the gap2 gene promoter activity in Giardia lamblia-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학)-
dc.contributor.googleauthorHye-won Yang-
dc.contributor.googleauthorJuri Kim-
dc.contributor.googleauthorTai-soon Yong-
dc.contributor.googleauthorSoon-jung Park-
dc.identifier.doi10.3347/kjp.2006.44.1.21-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01545-
dc.contributor.localIdA02424-
dc.relation.journalcodeJ01957-
dc.identifier.eissn1738-0006-
dc.identifier.pmid16514278-
dc.subject.keywordGiardia lamblia-
dc.subject.keywordgap2 gene expression-
dc.subject.keywordencystation-
dc.subject.keywordluciferase-
dc.subject.keywordtransfection-
dc.contributor.alternativeNamePark, Soon Jung-
dc.contributor.alternativeNameYong, Tai Soon-
dc.contributor.affiliatedAuthorPark, Soon Jung-
dc.contributor.affiliatedAuthorYong, Tai Soon-
dc.rights.accessRightsfree-
dc.citation.volume44-
dc.citation.number1-
dc.citation.startPage21-
dc.citation.endPage26-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF PARASITOLOGY, Vol.44(1) : 21-26, 2006-
dc.identifier.rimsid52268-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers

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