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Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage

Authors
 Kyu-lee Han  ;  Jongweon Lee  ;  Don-soo Kim  ;  Soon-jung Park  ;  Kyung-il Im  ;  Tai-soon Yong 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.44(1) : 15-20, 2006 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
2006
MeSH
Acanthamoeba/genetics* ; Acanthamoeba/pathogenicity* ; Amebiasis/mortality ; Amebiasis/parasitology* ; Animals ; Blotting, Northern/methods ; Brain/parasitology ; Cloning, Molecular/methods ; DNA, Complementary/biosynthesis ; DNA, Protozoan/biosynthesis ; DNA, Protozoan/physiology* ; Gene Expression Profiling/methods ; Gene Expression Regulation* ; Genes, Protozoan/genetics ; Mice ; Mice, Inbred ICR ; Molecular Sequence Data ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Serial Passage ; Up-Regulation ; Virulence/genetics
Keywords
Acanthamoeba culbertsoni ; free-living ameba ; mouse ; DDRT-PCR ; virulence
Abstract
Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from 5% to 70%. Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors.
Files in This Item:
T200600454.pdf Download
DOI
10.3347/kjp.2006.44.1.15
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Im, Kyung Il(임경일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109306
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