Cited 3 times in
Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage
DC Field | Value | Language |
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dc.contributor.author | 박순정 | - |
dc.contributor.author | 용태순 | - |
dc.contributor.author | 임경일 | - |
dc.date.accessioned | 2015-06-10T12:09:55Z | - |
dc.date.available | 2015-06-10T12:09:55Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109306 | - |
dc.description.abstract | Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from 5% to 70%. Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 15~20 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PARASITOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acanthamoeba/genetics* | - |
dc.subject.MESH | Acanthamoeba/pathogenicity* | - |
dc.subject.MESH | Amebiasis/mortality | - |
dc.subject.MESH | Amebiasis/parasitology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Blotting, Northern/methods | - |
dc.subject.MESH | Brain/parasitology | - |
dc.subject.MESH | Cloning, Molecular/methods | - |
dc.subject.MESH | DNA, Complementary/biosynthesis | - |
dc.subject.MESH | DNA, Protozoan/biosynthesis | - |
dc.subject.MESH | DNA, Protozoan/physiology* | - |
dc.subject.MESH | Gene Expression Profiling/methods | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Genes, Protozoan/genetics | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred ICR | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction/methods | - |
dc.subject.MESH | Serial Passage | - |
dc.subject.MESH | Up-Regulation | - |
dc.subject.MESH | Virulence/genetics | - |
dc.title | Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Kyu-lee Han | - |
dc.contributor.googleauthor | Jongweon Lee | - |
dc.contributor.googleauthor | Don-soo Kim | - |
dc.contributor.googleauthor | Soon-jung Park | - |
dc.contributor.googleauthor | Kyung-il Im | - |
dc.contributor.googleauthor | Tai-soon Yong | - |
dc.identifier.doi | 10.3347/kjp.2006.44.1.15 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01545 | - |
dc.contributor.localId | A02424 | - |
dc.contributor.localId | A03355 | - |
dc.relation.journalcode | J01957 | - |
dc.identifier.eissn | 1738-0006 | - |
dc.identifier.pmid | 16514277 | - |
dc.subject.keyword | Acanthamoeba culbertsoni | - |
dc.subject.keyword | free-living ameba | - |
dc.subject.keyword | mouse | - |
dc.subject.keyword | DDRT-PCR | - |
dc.subject.keyword | virulence | - |
dc.contributor.alternativeName | Park, Soon Jung | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.alternativeName | Im, Kyung Il | - |
dc.contributor.affiliatedAuthor | Park, Soon Jung | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Im, Kyung Il | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 44 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 15 | - |
dc.citation.endPage | 20 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PARASITOLOGY, Vol.44(1) : 15-20, 2006 | - |
dc.identifier.rimsid | 52267 | - |
dc.type.rims | ART | - |
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