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Synergistic Effect of Staphylococcus aureus and LPS on Silica-Induced Tumor Necrosis Factor Production in Macrophage Cell Line J774A.1

Authors
 Lee, Dong Hee  ;  Bong Joo Park  ;  Min Sub Lee  ;  Jae Bong Choi  ;  Jeong Koo Kim  ;  Jong Hoon Park  ;  Jong-Chul Park 
Citation
 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Vol.16(1) : 136-140, 2006 
Journal Title
 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 
ISSN
 1017-7825 
Issue Date
2006
Keywords
Synergistic effect ; Staphylococcus aureus ; lipopolysaccharide ; tumor necrosis factor ; silica ; macrophage ; phagocytosis
Abstract
In this study, we investigated the synergistic effects of Staphylococcus aureus extracts (membranes and walls) and lipopolysaccharide (LPS) derived from Escherichia coli on tumor necrosis factor (TNF) production in the pathogenesis of silica-induced inflammation. The synergistic induction of TNF by silica particles (<20 μm) in combination with either S. aureus extracts or LPS was examined in J774A.1 cell cultures. Media from the treated and untreated cell cultures were assayed for TNF, using the mouse WEHI 164 cell cytotoxicity assay and enzyme immunoassay. Cells exposed simultaneously to silica and 0.5 μg/ml S. aureus extracts (or 0.5 ng/ml of LPS) produced a significantly higher level of TNF than those produced by the inducer alone. Our results indicate that device-associated infections (or pyrogen contamination) could enhance inflammatory responses, because of particles produced by the wear of medical implants or particulate biomaterials used for clinical purposes.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109018
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