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Use of PLGA scaffold for mucociliary epithelium transfer in airway reconstruction: A preliminary study

 Chang-Hoon Kim  ;  Jung Ho Bae  ;  Seoyeon Son  ;  Jung-Hyun Kim  ;  Jeung-Gweon Lee  ;  Joo-Heon Yoon 
 ACTA OTO-LARYNGOLOGICA, Vol.126(6) : 594-599, 2006 
Journal Title
Issue Date
Absorbable Implants* ; Biocompatible Materials* ; Cell Differentiation/genetics ; Cells, Cultured ; Gene Expression/physiology ; Humans ; Lactic Acid* ; Membranes, Artificial* ; Microscopy, Electron, Scanning ; Mucin 5AC ; Mucins/genetics ; Mucociliary Clearance/genetics ; Mucociliary Clearance/physiology* ; Nasal Mucosa/transplantation* ; Polyglycolic Acid* ; Polylactic Acid-Polyglycolic Acid Copolymer ; Polymers* ; Reverse Transcriptase Polymerase Chain Reaction ; Tissue Engineering*
Cultured nasal epithelium ; mucociliary epithelium ; graft ; PLGA ; differentiation
Conclusion. A PLGA biodegradable membrane can be used as a scaffold for mucociliary epithelium transfer. Objectives. The aim of this study was to examine the usefulness of the PLGA membrane as a biodegradable scaffold for mucociliary epithelium transfer in order for it to be used as a substitute for a skin graft for restoring mucosal defects in the airway. Methods. A PLGA biodegradable membrane was synthesized using the immersion precipitation method, and morphologic characterization was carried out using scanning electron microscopy (SEM). The degradation test was performed by soaking the PLGA membrane in a culture medium, and the morphological changes were observed by SEM. Human nasal basal epithelial (HNBE) cells were cultured on the newly synthesized PLGA membrane, and the morphological changes were analyzed using SEM. The MUC5AC and MUC8 mRNA levels were analyzed by RT-PCR. Results. The PLGA membrane for the mucociliary epithelium transfer was successfully fabricated. It had a 24 mm diameter, a 50 µm thickness, and many pores with diameters of approximately 3 µm. The PLGA membrane began to degrade from 7 days after it was soaked in the culture medium. It rapidly degraded from 3 weeks and severe destruction of the pore structure was noted from 4 to 6 weeks of soaking. The HNBE cells were well differentiated into the mucociliary epithelium on the PLGA membrane both phenotypically and genotypically.
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1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
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