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Use of PLGA scaffold for mucociliary epithelium transfer in airway reconstruction: A preliminary study

DC FieldValueLanguage
dc.contributor.author김창훈-
dc.contributor.author윤주헌-
dc.contributor.author이정권-
dc.date.accessioned2015-06-10T11:59:04Z-
dc.date.available2015-06-10T11:59:04Z-
dc.date.issued2006-
dc.identifier.issn0001-6489-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/108977-
dc.description.abstractConclusion. A PLGA biodegradable membrane can be used as a scaffold for mucociliary epithelium transfer. Objectives. The aim of this study was to examine the usefulness of the PLGA membrane as a biodegradable scaffold for mucociliary epithelium transfer in order for it to be used as a substitute for a skin graft for restoring mucosal defects in the airway. Methods. A PLGA biodegradable membrane was synthesized using the immersion precipitation method, and morphologic characterization was carried out using scanning electron microscopy (SEM). The degradation test was performed by soaking the PLGA membrane in a culture medium, and the morphological changes were observed by SEM. Human nasal basal epithelial (HNBE) cells were cultured on the newly synthesized PLGA membrane, and the morphological changes were analyzed using SEM. The MUC5AC and MUC8 mRNA levels were analyzed by RT-PCR. Results. The PLGA membrane for the mucociliary epithelium transfer was successfully fabricated. It had a 24 mm diameter, a 50 µm thickness, and many pores with diameters of approximately 3 µm. The PLGA membrane began to degrade from 7 days after it was soaked in the culture medium. It rapidly degraded from 3 weeks and severe destruction of the pore structure was noted from 4 to 6 weeks of soaking. The HNBE cells were well differentiated into the mucociliary epithelium on the PLGA membrane both phenotypically and genotypically.-
dc.description.statementOfResponsibilityopen-
dc.format.extent594~599-
dc.relation.isPartOfACTA OTO-LARYNGOLOGICA-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAbsorbable Implants*-
dc.subject.MESHBiocompatible Materials*-
dc.subject.MESHCell Differentiation/genetics-
dc.subject.MESHCells, Cultured-
dc.subject.MESHGene Expression/physiology-
dc.subject.MESHHumans-
dc.subject.MESHLactic Acid*-
dc.subject.MESHMembranes, Artificial*-
dc.subject.MESHMicroscopy, Electron, Scanning-
dc.subject.MESHMucin 5AC-
dc.subject.MESHMucins/genetics-
dc.subject.MESHMucociliary Clearance/genetics-
dc.subject.MESHMucociliary Clearance/physiology*-
dc.subject.MESHNasal Mucosa/transplantation*-
dc.subject.MESHPolyglycolic Acid*-
dc.subject.MESHPolylactic Acid-Polyglycolic Acid Copolymer-
dc.subject.MESHPolymers*-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHTissue Engineering*-
dc.titleUse of PLGA scaffold for mucociliary epithelium transfer in airway reconstruction: A preliminary study-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학)-
dc.contributor.googleauthorChang-Hoon Kim-
dc.contributor.googleauthorJung Ho Bae-
dc.contributor.googleauthorSeoyeon Son-
dc.contributor.googleauthorJung-Hyun Kim-
dc.contributor.googleauthorJeung-Gweon Lee-
dc.contributor.googleauthorJoo-Heon Yoon-
dc.identifier.doi10.1080/00016480500443375-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01050-
dc.contributor.localIdA02604-
dc.contributor.localIdA03095-
dc.relation.journalcodeJ00028-
dc.identifier.eissn1651-2251-
dc.identifier.pmid16720443-
dc.identifier.urlhttp://informahealthcare.com/doi/abs/10.1080/00016480500443375-
dc.subject.keywordCultured nasal epithelium-
dc.subject.keywordmucociliary epithelium-
dc.subject.keywordgraft-
dc.subject.keywordPLGA-
dc.subject.keyworddifferentiation-
dc.contributor.alternativeNameKim, Chang Hoon-
dc.contributor.alternativeNameYoon, Joo Heon-
dc.contributor.alternativeNameLee, Jeung Gweon-
dc.contributor.affiliatedAuthorKim, Chang Hoon-
dc.contributor.affiliatedAuthorYoon, Joo Heon-
dc.contributor.affiliatedAuthorLee, Jeung Gweon-
dc.rights.accessRightsnot free-
dc.citation.volume126-
dc.citation.number6-
dc.citation.startPage594-
dc.citation.endPage599-
dc.identifier.bibliographicCitationACTA OTO-LARYNGOLOGICA, Vol.126(6) : 594-599, 2006-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

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