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N-acetylcysteine stimulates osteoblastic differentiation of mouse calvarial cells

Authors
 Ji Hae Jun  ;  Sun-Hwan Lee  ;  Han Bok Kwak  ;  Zang Hee Lee  ;  Sang-Beum Seo  ;  Kyung Mi Woo  ;  Hyun-Mo Ryoo  ;  Gwan-Shik Kim  ;  Jeong-Hwa Baek 
Citation
 JOURNAL OF CELLULAR BIOCHEMISTRY, Vol.103(4) : 1246-1255, 2008 
Journal Title
 JOURNAL OF CELLULAR BIOCHEMISTRY 
ISSN
 0730-2312 
Issue Date
2008
MeSH
Acetylcysteine/pharmacology* ; Alkaline Phosphatase/metabolism ; Animals ; Antioxidants/pharmacology* ; Bacterial Proteins/pharmacology ; Bacterial Toxins/pharmacology ; Bone Morphogenetic Proteins/metabolism ; Caffeic Acids/pharmacology ; Cell Differentiation ; Cells, Cultured ; Glutathione/metabolism ; Mice ; Osteoblasts/cytology* ; Osteogenesis ; Phenylethyl Alcohol/analogs & derivatives ; Phenylethyl Alcohol/pharmacology ; Skull/cytology* ; rho GTP-Binding Proteins/metabolism
Keywords
N-acetylcysteine ; osteoblast differentiation ; glutathione ; inhibition of RhoA activity
Abstract
Estrogen deficiency causes osteoporosis via increased generation of reactive oxygen species (ROS), and thus, antioxidants may prove to be the effective therapeutic candidates. We examined the effects of the antioxidant N-acetylcysteine (NAC) on osteoblastic differentiation in mouse calvarial cells. NAC (10-30 mM) enhanced alkaline phosphatase activity, mRNA expression of osteoblast differentiation-associated genes and mineralized nodule formation. It also increased expression of bone morphogenetic proteins-2, -4, and -7. The osteogenic activity of NAC was partially reduced by inhibition of glutathione synthesis. Since caffeic acid phenethyl ester did not stimulate osteoblast differentiation, it is unlikely that ROS scavenging activity of NAC is sufficient for osteogenic activity. We observed that NAC suppressed small GTPase RhoA activity and activation of RhoA by Pasteurella multocida toxin suppressed the osteogenic activity of NAC. These results suggest that NAC might exert its osteogenic activity via increased glutathione synthesis and inhibition of RhoA activation
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jcb.21508/abstract
DOI
10.1002/jcb.21508
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Jun, Ji Hae(전지혜) ORCID logo https://orcid.org/0000-0002-8080-0715
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108633
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