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N-acetylcysteine stimulates osteoblastic differentiation of mouse calvarial cells

DC FieldValueLanguage
dc.contributor.author전지혜-
dc.date.accessioned2015-05-19T17:41:34Z-
dc.date.available2015-05-19T17:41:34Z-
dc.date.issued2008-
dc.identifier.issn0730-2312-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/108633-
dc.description.abstractEstrogen deficiency causes osteoporosis via increased generation of reactive oxygen species (ROS), and thus, antioxidants may prove to be the effective therapeutic candidates. We examined the effects of the antioxidant N-acetylcysteine (NAC) on osteoblastic differentiation in mouse calvarial cells. NAC (10-30 mM) enhanced alkaline phosphatase activity, mRNA expression of osteoblast differentiation-associated genes and mineralized nodule formation. It also increased expression of bone morphogenetic proteins-2, -4, and -7. The osteogenic activity of NAC was partially reduced by inhibition of glutathione synthesis. Since caffeic acid phenethyl ester did not stimulate osteoblast differentiation, it is unlikely that ROS scavenging activity of NAC is sufficient for osteogenic activity. We observed that NAC suppressed small GTPase RhoA activity and activation of RhoA by Pasteurella multocida toxin suppressed the osteogenic activity of NAC. These results suggest that NAC might exert its osteogenic activity via increased glutathione synthesis and inhibition of RhoA activation-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF CELLULAR BIOCHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAcetylcysteine/pharmacology*-
dc.subject.MESHAlkaline Phosphatase/metabolism-
dc.subject.MESHAnimals-
dc.subject.MESHAntioxidants/pharmacology*-
dc.subject.MESHBacterial Proteins/pharmacology-
dc.subject.MESHBacterial Toxins/pharmacology-
dc.subject.MESHBone Morphogenetic Proteins/metabolism-
dc.subject.MESHCaffeic Acids/pharmacology-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCells, Cultured-
dc.subject.MESHGlutathione/metabolism-
dc.subject.MESHMice-
dc.subject.MESHOsteoblasts/cytology*-
dc.subject.MESHOsteogenesis-
dc.subject.MESHPhenylethyl Alcohol/analogs & derivatives-
dc.subject.MESHPhenylethyl Alcohol/pharmacology-
dc.subject.MESHSkull/cytology*-
dc.subject.MESHrho GTP-Binding Proteins/metabolism-
dc.titleN-acetylcysteine stimulates osteoblastic differentiation of mouse calvarial cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorJi Hae Jun-
dc.contributor.googleauthorSun-Hwan Lee-
dc.contributor.googleauthorHan Bok Kwak-
dc.contributor.googleauthorZang Hee Lee-
dc.contributor.googleauthorSang-Beum Seo-
dc.contributor.googleauthorKyung Mi Woo-
dc.contributor.googleauthorHyun-Mo Ryoo-
dc.contributor.googleauthorGwan-Shik Kim-
dc.contributor.googleauthorJeong-Hwa Baek-
dc.identifier.doi10.1002/jcb.21508-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03551-
dc.relation.journalcodeJ01303-
dc.identifier.eissn1097-4644-
dc.identifier.pmid17979115-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jcb.21508/abstract-
dc.subject.keywordN-acetylcysteine-
dc.subject.keywordosteoblast differentiation-
dc.subject.keywordglutathione-
dc.subject.keywordinhibition of RhoA activity-
dc.contributor.alternativeNameJun, Ji Hae-
dc.contributor.affiliatedAuthorJun, Ji Hae-
dc.rights.accessRightsnot free-
dc.citation.volume103-
dc.citation.number4-
dc.citation.startPage1246-
dc.citation.endPage1255-
dc.identifier.bibliographicCitationJOURNAL OF CELLULAR BIOCHEMISTRY, Vol.103(4) : 1246-1255, 2008-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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