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In Utero Exposure to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Affects the Development of Reproductive System in Mouse

 Mei Hua Jin  ;  Hae Kyung Ko  ;  Chang Hee Hong  ;  Sang Won Han 
 YONSEI MEDICAL JOURNAL, Vol.49(5) : 843-850, 2008 
Journal Title
Issue Date
Animals ; Apoptosis/drug effects ; Cell Proliferation ; Embryonic Development/drug effects* ; Environmental Pollutants/toxicity* ; Female ; Male ; Maternal Exposure* ; Mice ; Mice, Inbred C57BL ; Organ Size/drug effects ; Polychlorinated Dibenzodioxins/toxicity* ; Pregnancy ; Receptors, Androgen/metabolism ; Testis/drug effects* ; Testis/embryology ; Testis/pathology
2,3,7,8-Tetrachlorodibenzo-p-Dioxin ; androgen receptor ; testis ; apoptosis ; proliferation
PURPOSE: Exposure of male reproductive organs to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) has been reported to cause developmental changes. In this study, we evaluated the effects of in utero TCDD exposure on male reproductive development. MATERIALS AND METHODS: Pregnant C57BL/6 mice were administered a single intraperitoneal injection of TCDD (1microgram/kg) on gestation day (GD) 15. The offspring were examined in the immature stage on postnatal day (PND) 30 and in the mature stage on PND 60. The testes were examined for histological changes, androgen receptor (AR), proliferating cell nuclear antigen (PCNA) and apoptosis following the measurement of morphological changes. RESULTS: Anogenital distance (AGD) and testis weights were reduced by TCDD exposure both on PND 30 and PND 60 while body weights and length of male offspring were not affected by TCDD. The regular sperm developmental stage was impaired with TCDD treatment on PND 30. However, no difference was found between the control group and TCDD groups on PND 60. Simultaneously, the expression of AR was also reduced on PND 30, while it was increased on PND 60 compared with the control group. The expression of PCNA was decreased whereas apoptosis was not affected by TCDD both on PND 30 and PND 60. CONCLUSION: These results suggest that in utero exposure to TCDD influences the development of testes by inhibiting the expression of AR and PCNA. Moreover, the adverse effects of TCDD on male offspring reduced over time
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1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Ko, Hae Kyung(고혜경)
Jin, Mei Hua(김매화)
Han, Sang Won(한상원) ORCID logo https://orcid.org/0000-0003-0941-1300
Hong, Chang Hee(홍창희) ORCID logo https://orcid.org/0000-0002-0946-7702
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