175 227

Cited 27 times in

Functional expression of CXCR4 in somatotrophs: CXCL12 activates GH gene, GH production and secretion, and cellular proliferation

Authors
 Yongho Lee  ;  Jeong Mo Kim  ;  Eun Jig Lee 
Citation
 JOURNAL OF ENDOCRINOLOGY, Vol.199(2) : 191-199, 2008 
Journal Title
 JOURNAL OF ENDOCRINOLOGY 
ISSN
 0022-0795 
Issue Date
2008
MeSH
Animals ; Blotting, Western ; Cell Line, Tumor ; Cell Proliferation* ; Cells, Cultured ; Chemokine CXCL12/chemistry ; Chemokine CXCL12/metabolism ; Chemokine CXCL12/physiology* ; Fluorescent Antibody Technique ; Gene Expression/physiology ; Growth Hormone/genetics ; Growth Hormone/metabolism* ; Immunohistochemistry ; Male ; Protein Binding/genetics ; Protein Binding/physiology ; Rats ; Rats, Sprague-Dawley ; Receptors, CXCR4/chemistry ; Receptors, CXCR4/metabolism ; Receptors, CXCR4/physiology* ; Reverse Transcriptase Polymerase Chain Reaction ; Somatotrophs/metabolism*
Keywords
Animals ; Blotting, Western ; Cell Line, Tumor ; Cell Proliferation* ; Cells, Cultured ; Chemokine CXCL12/chemistry ; Chemokine CXCL12/metabolism ; Chemokine CXCL12/physiology* ; Fluorescent Antibody Technique ; Gene Expression/physiology ; Growth Hormone/genetics ; Growth Hormone/metabolism* ; Immunohistochemistry ; Male ; Protein Binding/genetics ; Protein Binding/physiology ; Rats ; Rats, Sprague-Dawley ; Receptors, CXCR4/chemistry ; Receptors, CXCR4/metabolism ; Receptors, CXCR4/physiology* ; Reverse Transcriptase Polymerase Chain Reaction ; Somatotrophs/metabolism*
Abstract
The interaction of chemokine (C-X-C motif) ligand 12 (CXCL12) and its receptor CXCR4 may play an important role in the regulation of anterior pituitary function. In this study, we investigated the expression of CXCL12 and CXCR4 and their role in normal rat pituitary and GH-producing GH3 tumor cell line. RT-PCR analysis and immunohistochemistry revealed that CXCR4 was expressed in normal rat anterior pituitary and GH3 tumor cells. Double immunofluorescent staining showed the complete colocalization of CXCR4 with GH in rat pituitary, indicating that CXCR4 is specifically expressed in rat somatotrophs. Using rat primary pituitary cell cultures and GH releasing hormone receptor expressing stable GH3 cells (GH3-GHRHR), we evaluated the function of CXCL12 compared with GHRH. CXCL12 stimulated GH gene activation in both primary rat anterior pituitary cells and GH3-GHRHR cells. CXCL12 also stimulated GH secretion from primary rat pituitary cells in a dose-dependant manner. BrdU incorporation was increased in response to CXCL12 addition in GH3 cell culture, indicating CXCL12-induced cell proliferation. CXCL12-dependent phosphorylation of ERK1/2 was also confirmed by western blot analysis, supporting the evidence that MAPK is an intracellular mediator of CXCL12/CXCR4 interaction in GH3 cell proliferation. In conclusion, these results indicate that CXCL12/CXCR4 interaction plays an important role in GH production, secretion, and the proliferation of somatotrophs
Files in This Item:
T200805068.pdf Download
DOI
10.1677/JOE-08-0250
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jeong Mo(김정모)
Lee, Yong Ho(이용호) ORCID logo https://orcid.org/0000-0002-6219-4942
Lee, Eun Jig(이은직) ORCID logo https://orcid.org/0000-0002-9876-8370
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108339
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse