Cited 43 times in
Functional expression of CXCR4 in somatotrophs: CXCL12 activates GH gene, GH production and secretion, and cellular proliferation
DC Field | Value | Language |
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dc.contributor.author | 김정모 | - |
dc.contributor.author | 이용호 | - |
dc.contributor.author | 이은직 | - |
dc.date.accessioned | 2015-05-19T17:32:28Z | - |
dc.date.available | 2015-05-19T17:32:28Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0022-0795 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108339 | - |
dc.description.abstract | The interaction of chemokine (C-X-C motif) ligand 12 (CXCL12) and its receptor CXCR4 may play an important role in the regulation of anterior pituitary function. In this study, we investigated the expression of CXCL12 and CXCR4 and their role in normal rat pituitary and GH-producing GH3 tumor cell line. RT-PCR analysis and immunohistochemistry revealed that CXCR4 was expressed in normal rat anterior pituitary and GH3 tumor cells. Double immunofluorescent staining showed the complete colocalization of CXCR4 with GH in rat pituitary, indicating that CXCR4 is specifically expressed in rat somatotrophs. Using rat primary pituitary cell cultures and GH releasing hormone receptor expressing stable GH3 cells (GH3-GHRHR), we evaluated the function of CXCL12 compared with GHRH. CXCL12 stimulated GH gene activation in both primary rat anterior pituitary cells and GH3-GHRHR cells. CXCL12 also stimulated GH secretion from primary rat pituitary cells in a dose-dependant manner. BrdU incorporation was increased in response to CXCL12 addition in GH3 cell culture, indicating CXCL12-induced cell proliferation. CXCL12-dependent phosphorylation of ERK1/2 was also confirmed by western blot analysis, supporting the evidence that MAPK is an intracellular mediator of CXCL12/CXCR4 interaction in GH3 cell proliferation. In conclusion, these results indicate that CXCL12/CXCR4 interaction plays an important role in GH production, secretion, and the proliferation of somatotrophs | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 191~199 | - |
dc.relation.isPartOf | JOURNAL OF ENDOCRINOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Proliferation* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Chemokine CXCL12/chemistry | - |
dc.subject.MESH | Chemokine CXCL12/metabolism | - |
dc.subject.MESH | Chemokine CXCL12/physiology* | - |
dc.subject.MESH | Fluorescent Antibody Technique | - |
dc.subject.MESH | Gene Expression/physiology | - |
dc.subject.MESH | Growth Hormone/genetics | - |
dc.subject.MESH | Growth Hormone/metabolism* | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Protein Binding/genetics | - |
dc.subject.MESH | Protein Binding/physiology | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Receptors, CXCR4/chemistry | - |
dc.subject.MESH | Receptors, CXCR4/metabolism | - |
dc.subject.MESH | Receptors, CXCR4/physiology* | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Somatotrophs/metabolism* | - |
dc.title | Functional expression of CXCR4 in somatotrophs: CXCL12 activates GH gene, GH production and secretion, and cellular proliferation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Yongho Lee | - |
dc.contributor.googleauthor | Jeong Mo Kim | - |
dc.contributor.googleauthor | Eun Jig Lee | - |
dc.identifier.doi | 10.1677/JOE-08-0250 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00881 | - |
dc.contributor.localId | A02989 | - |
dc.contributor.localId | A03050 | - |
dc.relation.journalcode | J01392 | - |
dc.identifier.eissn | 1479-6805 | - |
dc.identifier.pmid | 18753332 | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Blotting, Western | - |
dc.subject.keyword | Cell Line, Tumor | - |
dc.subject.keyword | Cell Proliferation* | - |
dc.subject.keyword | Cells, Cultured | - |
dc.subject.keyword | Chemokine CXCL12/chemistry | - |
dc.subject.keyword | Chemokine CXCL12/metabolism | - |
dc.subject.keyword | Chemokine CXCL12/physiology* | - |
dc.subject.keyword | Fluorescent Antibody Technique | - |
dc.subject.keyword | Gene Expression/physiology | - |
dc.subject.keyword | Growth Hormone/genetics | - |
dc.subject.keyword | Growth Hormone/metabolism* | - |
dc.subject.keyword | Immunohistochemistry | - |
dc.subject.keyword | Male | - |
dc.subject.keyword | Protein Binding/genetics | - |
dc.subject.keyword | Protein Binding/physiology | - |
dc.subject.keyword | Rats | - |
dc.subject.keyword | Rats, Sprague-Dawley | - |
dc.subject.keyword | Receptors, CXCR4/chemistry | - |
dc.subject.keyword | Receptors, CXCR4/metabolism | - |
dc.subject.keyword | Receptors, CXCR4/physiology* | - |
dc.subject.keyword | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.keyword | Somatotrophs/metabolism* | - |
dc.contributor.alternativeName | Kim, Jeong Mo | - |
dc.contributor.alternativeName | Lee, Yong Ho | - |
dc.contributor.alternativeName | Lee, Eun Jig | - |
dc.contributor.affiliatedAuthor | Kim, Jeong Mo | - |
dc.contributor.affiliatedAuthor | Lee, Yong Ho | - |
dc.contributor.affiliatedAuthor | Lee, Eun Jig | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 199 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 191 | - |
dc.citation.endPage | 199 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ENDOCRINOLOGY, Vol.199(2) : 191-199, 2008 | - |
dc.identifier.rimsid | 35497 | - |
dc.type.rims | ART | - |
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