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Differentially expressed proteins in cerulein-stimulated pancreatic acinar cells: implication for acute pancreatitis.

Authors
 Ji Hoon Yu  ;  Jeong Yeon Seo  ;  Kyung Hwan Kim  ;  Hyeyoung Kim 
Citation
 INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol.40(3) : 503-516, 2008 
Journal Title
 INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 
ISSN
 1357-2725 
Issue Date
2008
MeSH
Acute Disease ; Animals ; Cell Line, Tumor ; Ceruletide ; Disease Models, Animal ; Down-Regulation ; Electrophoresis, Gel, Two-Dimensional ; Pancreatitis/chemically induced ; Pancreatitis/metabolism* ; Protein Array Analysis ; Proteins/metabolism* ; Rats ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Up-Regulation
Keywords
Cerulein ; Pancreatitis ; AR42J cells ; Proteomic analysis
Abstract
The proteins expressed in pancreatic acinar cells during the initiation of acute pancreatitis may determine the severity of the disease. Cerulein pancreatitis is one of the best characterized models for acute pancreatitis. Present study aims to determine the differentially expressed proteins in cerulein-stimulated pancreatic acinar cells as an in vitro model for acute pancreatitis. Rat pancreatic acinar AR42J cells were treated with 10(-8)M cerulein for 12h. The protein patterns separated by two-dimensional electrophoresis using pH gradients of 5-8 were compared between the cells treated without cerulein and those with cerulein. The changed proteins were conclusively identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of the peptide digests. As a result, 10 proteins (Orp150 protein, protein disulfide isomerase related protein, dnaK-type molecular chaperone hsp72-ps1, mitochondrial glutamate dehydrogenase, similar to chaperonin containing TCP-1 beta subunit, RuvB-like protein 1, heterogeneous nuclear ribonucleoprotein H1, aldehyde reductase 1, triosephosphate isomerase 1, peroxiredoxin 2) were up-regulated while four proteins (vasolin-containing protein, 78 kDa glucose-regulated protein precursor, heat shock protein 8, adenosylhomocysteinase) were down-regulated by cerulein in pancreatic acinar AR42J cells. These proteins are related to chaperone, cell defense mechanism against oxidative stress or DNA damage, anti-apoptosis and energy generation. The differentially expressed proteins by ceruein share their functional roles in pancreatic acinar cells, suggesting the possible involvement of oxidative stress, DNA damage, and anti-apoptosis in pathogenesis of acute pancreatitis. Proteins involved in cellular defense mechanism and energy production may protect pancreatic acinar cells during the development of pancreatitis.
Full Text
http://www.sciencedirect.com/science/article/pii/S1357272507003019
DOI
10.1016/j.biocel.2007.09.001
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
Kim, Hye Young(김혜영)
Seo, Jeong Yeon(서정연)
Yu, Ji Hoon(유지훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108289
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