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Real-time Imaging of Inositol 1,4,5-trisphosphate Movement in Mouse Salivary Gland Cells

Authors
 Jeong Hee Hong  ;  Syng-Ill Lee  ;  Dong Min Shin 
Citation
 International Journal of Oral Biology, Vol.33(4) : 125-129, 2008 
Journal Title
 International Journal of Oral Biology 
ISSN
 1226-7155 
Issue Date
2008
Keywords
inositol 1,4,5-trisphosphate imaging ; green fluorescent protein ; transgenic mouse ; parotid gland ; submandibular gland
Abstract
Inositol 1,4,5-trisphosphate () plays an important role in the release of from intracellular stores into the cytoplasm in a variety of cell types. translocation dynamics have been studied in response to many types of cell signals. However, the dynamics of cytosolic in salivary acinar cells are unclear. A green fluorescent protein (GFP)-tagged pleckstrin homology domain (PHD) was constructed and introduced into a phospholipase C (PLC ) transgenic mouse, and then the salivary acinar cells were isolated. GFP-PHD was heterogeneously localized at the plasma membrane and intracellular organelles in submandibular gland and parotid gland cells. Application of trypsin, a G protein-coupled receptor activator, to the two types of cells caused an increase in GFP fluorescence in the cell cytoplasm. The observed time course of trypsin-evoked movement in acinar cells was independent of cell polarity, and the fluorescent label showed an immediate increase throughout the cells. These results suggest that GFP-PHD in many tissues of transgenic mice, including non-cultured primary cells, can be used as a model for examination of intracellular dynamics.
Files in This Item:
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Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Hong, Jeong Hee(홍정희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107879
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