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Real-time Imaging of Inositol 1,4,5-trisphosphate Movement in Mouse Salivary Gland Cells
DC Field | Value | Language |
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dc.contributor.author | 신동민 | - |
dc.contributor.author | 홍정희 | - |
dc.date.accessioned | 2015-05-19T17:17:52Z | - |
dc.date.available | 2015-05-19T17:17:52Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/107879 | - |
dc.description.abstract | Inositol 1,4,5-trisphosphate () plays an important role in the release of from intracellular stores into the cytoplasm in a variety of cell types. translocation dynamics have been studied in response to many types of cell signals. However, the dynamics of cytosolic in salivary acinar cells are unclear. A green fluorescent protein (GFP)-tagged pleckstrin homology domain (PHD) was constructed and introduced into a phospholipase C (PLC ) transgenic mouse, and then the salivary acinar cells were isolated. GFP-PHD was heterogeneously localized at the plasma membrane and intracellular organelles in submandibular gland and parotid gland cells. Application of trypsin, a G protein-coupled receptor activator, to the two types of cells caused an increase in GFP fluorescence in the cell cytoplasm. The observed time course of trypsin-evoked movement in acinar cells was independent of cell polarity, and the fluorescent label showed an immediate increase throughout the cells. These results suggest that GFP-PHD in many tissues of transgenic mice, including non-cultured primary cells, can be used as a model for examination of intracellular dynamics. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 125~129 | - |
dc.relation.isPartOf | International Journal of Oral Biology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Real-time Imaging of Inositol 1,4,5-trisphosphate Movement in Mouse Salivary Gland Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Jeong Hee Hong | - |
dc.contributor.googleauthor | Syng-Ill Lee | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A04434 | - |
dc.relation.journalcode | J01144 | - |
dc.identifier.pmid | inositol 1,4,5-trisphosphate imaging; green fluorescent protein; transgenic mouse; parotid gland; submandibular gland | - |
dc.subject.keyword | inositol 1,4,5-trisphosphate imaging | - |
dc.subject.keyword | green fluorescent protein | - |
dc.subject.keyword | transgenic mouse | - |
dc.subject.keyword | parotid gland | - |
dc.subject.keyword | submandibular gland | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Hong, Jeong Hee | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Hong, Jeong Hee | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 33 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 125 | - |
dc.citation.endPage | 129 | - |
dc.identifier.bibliographicCitation | International Journal of Oral Biology, Vol.33(4) : 125-129, 2008 | - |
dc.identifier.rimsid | 34748 | - |
dc.type.rims | ART | - |
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