6 347

Cited 8 times in

Identification and characterization of a mitochondrial iron-superoxide dismutase of Cryptosporidium parvum

Authors
 Jung-Mi Kang  ;  Hyeng-Il Cheun  ;  Juri Kim  ;  Sung-Ung Moon  ;  Soon-Jung Park  ;  Tong-Soo Kim  ;  Woon-Mok Sohn  ;  Byoung-Kuk Na 
Citation
 PARASITOLOGY RESEARCH, Vol.103(4) : 787-795, 2008 
Journal Title
 PARASITOLOGY RESEARCH 
ISSN
 0932-0113 
Issue Date
2008
MeSH
Amino Acid Motifs ; Animals ; Binding Sites ; Cloning, Molecular ; Conserved Sequence ; Cryptosporidium parvum/enzymology* ; Cryptosporidium parvum/genetics* ; DNA, Protozoan/chemistry ; DNA, Protozoan/genetics ; Enzyme Inhibitors/pharmacology ; Enzyme Stability ; Hydrogen Peroxide/pharmacology ; Hydrogen-Ion Concentration ; Mitochondrial Proteins/chemistry ; Mitochondrial Proteins/genetics* ; Mitochondrial Proteins/metabolism ; Molecular Sequence Data ; Protein Sorting Signals ; Protein Structure, Secondary ; Protein Transport ; Protozoan Proteins/chemistry ; Protozoan Proteins/genetics* ; Protozoan Proteins/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Sequence Alignment ; Sequence Analysis, DNA ; Superoxide Dismutase/chemistry ; Superoxide Dismutase/genetics* ; Superoxide Dismutase/metabolism
Keywords
Deduce Amino Acid Sequence ; Cryptosporidiosis ; Cryptosporidium Parvum ; Basic Amino Acid Residue ; Mitochondrial Iron
Abstract
Cryptosporidium parvum is an intracellular protozoan parasite that causes cryptosporidiosis in mammals. In this study, we identified a gene encoding mitochondrial iron-superoxide dismutase of C. parvum (Cp-mtSOD) and characterized biochemical properties of the recombinant protein. Multiple sequence alignment of the deduced amino acid sequence of Cp-mtSOD with those of previously reported iron-containing SODs (Fe-SODs) from other protozoan parasites showed that Cp-mtSOD shares common metal-binding residues and motifs that were conserved in Fe-SODs. However, the N-terminal 26-amino acid residues of Cp-mtSOD did not show sequence identities to any other Fe-SOD sequences. Further analysis of the N-terminal presequence of Cp-mtSOD suggested that it shares common physiochemical characteristics found in mitochondria targeting sequences and predicted localization of Cp-mtSOD in the mitochondria. The recombinant Cp-mtSOD showed typical biochemical properties with other characterized Fe-SODs, including molecular structure, broad pH optimum, and sensitivity to hydrogen peroxide.
Full Text
http://link.springer.com/article/10.1007%2Fs00436-008-1041-1
DOI
10.1007/s00436-008-1041-1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107668
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links