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Characterization and TCR variable region gene use of mouse resident nasal gammadelta T lymphocytes

Authors
 Chang-Hoon Kim  ;  Deborah A. Witherden  ;  Wendy L. Havran 
Citation
 JOURNAL OF LEUKOCYTE BIOLOGY, Vol.84(5) : 1259-1263, 2008 
Journal Title
 JOURNAL OF LEUKOCYTE BIOLOGY 
ISSN
 0741-5400 
Issue Date
2008
MeSH
Animals ; Flow Cytometry ; Immunoglobulin Variable Region* ; Mice ; Nasal Mucosa/immunology* ; Receptors, Antigen, T-Cell/genetics* ; Receptors, Antigen, T-Cell/immunology ; Receptors, Antigen, T-Cell, alpha-beta/genetics ; Receptors, Antigen, T-Cell, alpha-beta/immunology ; Receptors, Antigen, T-Cell, gamma-delta/genetics* ; Receptors, Antigen, T-Cell, gamma-delta/immunology* ; Reverse Transcriptase Polymerase Chain Reaction ; Spleen/immunology ; T-Lymphocytes/immunology*
Keywords
resident nasal lymphocyte ; nasal intraepithelial lymphocyte ; nasal lamina propria lymphocyte
Abstract
Tissue-resident gammadelta T lymphocytes, such as dendritic epidermal T cells, intestinal intraepithelial lymphocytes (IEL), and resident pulmonary lymphocytes, are known to support local tissue homeostasis and host defense. Inhaled antigens, toxins, and microorganisms first interact with the immune system through contact with the nasal mucosa. Herein, we characterized two populations of resident nasal lymphocytes (RNL) that are present in the nasal mucosa: nasal IEL (nIEL) and nasal lamina propria lymphocytes (nLPL). gammadelta TCR+ and alphabeta TCR+ nIEL and nLPL were detected by immunofluorescent staining. Mononuclear cells (5-15%) were CD3+ RNL by FACS analysis. Among the CD3+ RNL, 20-30% were GL3+ gammadelta T cells, which were double-negative for CD4 and CD8 and predominantly expressed a Vgamma4/Vdelta1 TCR. These results demonstrate that RNL might be crucial for the host defense and tissue homeostasis in the nasal mucosa.
Files in This Item:
T200801205.pdf Download
DOI
18667688
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107494
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