Gene expression patterns in glucose-stimulated podocytes
Authors
Seung Hyeok Han ; Sanghwa Yang ; Dong Sub Jung ; Jin Ji Li ; Jin Ju Kim ; Seung Jae Kwak ; Dong Ki Kim ; Sung Jin Moon ; Jung Eun Lee ; Dae-Suk Han ; Shin-Wook Kang
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.370(3) : 514-518, 2008
To explore the mechanisms of podocyte injury under diabetic conditions, we performed an expression profile in glucose-stimulated podocytes. Differential gene expression profiles between conditionally immortalized mouse podocytes cultured in medium containing 5.6 and 30 mM glucose were measured with oligonucleotide microarrays. Of the genes identified, heme oxygenase-1, vascular endothelial growth factor-A, and thrombospondin-1 showed a consistently increased pattern, whereas angiotensin-converting enzyme-2 and peroxisomal proliferator activator receptor-gamma were down-regulated. These results were validated using real-time PCR and western blotting in podocytes, and with immunohistochemistry on renal tissues from streptozotocin-induced diabetic rats. Not only is this the first report of gene expression profiling of podocyte injury under diabetic conditions, but the identified genes are promising targets for future diabetes research.