3 355

Cited 18 times in

Gene expression patterns in glucose-stimulated podocytes

Authors
 Seung Hyeok Han  ;  Sanghwa Yang  ;  Dong Sub Jung  ;  Jin Ji Li  ;  Jin Ju Kim  ;  Seung Jae Kwak  ;  Dong Ki Kim  ;  Sung Jin Moon  ;  Jung Eun Lee  ;  Dae-Suk Han  ;  Shin-Wook Kang 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.370(3) : 514-518, 2008 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2008
MeSH
Animals ; Blotting, Western ; Cells, Cultured ; Diabetic Nephropathies/genetics* ; Diabetic Nephropathies/pathology ; Disease Models, Animal ; Gene Expression/drug effects ; Gene Expression Profiling* ; Glucose/metabolism* ; Glucose/pharmacology ; Mice ; Podocytes/drug effects ; Podocytes/metabolism* ; Podocytes/pathology ; Rats ; Reverse Transcriptase Polymerase Chain Reaction
Keywords
Podocyte ; Gene expression ; Diabetic nephropathy
Abstract
To explore the mechanisms of podocyte injury under diabetic conditions, we performed an expression profile in glucose-stimulated podocytes. Differential gene expression profiles between conditionally immortalized mouse podocytes cultured in medium containing 5.6 and 30 mM glucose were measured with oligonucleotide microarrays. Of the genes identified, heme oxygenase-1, vascular endothelial growth factor-A, and thrombospondin-1 showed a consistently increased pattern, whereas angiotensin-converting enzyme-2 and peroxisomal proliferator activator receptor-gamma were down-regulated. These results were validated using real-time PCR and western blotting in podocytes, and with immunohistochemistry on renal tissues from streptozotocin-induced diabetic rats. Not only is this the first report of gene expression profiling of podocyte injury under diabetic conditions, but the identified genes are promising targets for future diabetes research.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X08006220
DOI
10.1016/j.bbrc.2008.03.121
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Cancer Metastasis Research Center (암전이연구센터) > 1. Journal Papers
Yonsei Authors
Kang, Shin Wook(강신욱) ORCID logo https://orcid.org/0000-0002-5677-4756
Kim, Dong Ki(김동기)
Moon, Sung Jin(문성진)
Yang, Sang Hwa(양상화)
Lee, Jung Eun(이정은) ORCID logo https://orcid.org/0000-0003-0917-2872
Han, Dae Suk(한대석)
Han, Seung Hyeok(한승혁) ORCID logo https://orcid.org/0000-0001-7923-5635
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106858
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse