The effects of Hydroxyapatite/Chitosan (H/C) Block scaffold in One-wall Intrabony Defect of Beagle Dogs

Abstract

The effect of Hydroxyapatite/Chitosan (H/C) block scaffold and HA/β-TCP (H/T) particle on the regeneration was evaluated in one-wall intrabony defects in beagle dogs. Six male beagle dogs were used in this study. H/C block scaffold was manufactured by freeze-dried method. One-wall intrabony periodontal defects (4 × 4 mm) were surgically created. Prepared defects were randomly assigned and treated as follows. In surgical control group (C) the defects were not filled. In HA/β-TCP group (H/T), defects were filled with HA/β-TCP particle bone graft material. In HA/Chitosan block group (H/C), defects were filled with HA/Chitosan Block scaffold. In all groups, Mean values of regenerated bone, regenerated cementum, connective tissue, and epithelium were measured. H/C group showed less inflammatory reaction than other groups. It revealed good biocompatibility. This group also showed better cementum regeneration than any other group. However, due to the relatively rapid resorption of chitosan, space maintenance for regeneration seems to have been compromised. Bone regeneration was affected adversely with this rapid resorption. More favorable bone regeneration was found in H/T group because proper space was maintained with Β-TCP