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CD34-인체 제대혈 유래 단핵구 세포가 피부창상 치유 촉진에 미치는 영향

 박상은  ;  유대현  ;  탁관철  ;  나동균 
 Journal of Korean Burn Society (대한화상학회지), Vol.12(1) : 38-42, 2009 
Journal Title
Journal of Korean Burn Society(대한화상학회지)
Issue Date
Cord blood stem cell transplantation ; Wound healing ; Leukocytes ; Mononuclear
Purpose: Cord blood stem cells are easily obtainable and have characteristics of higher proliferative potential and lower immunogenicity and with benefits in allogenic transplantation for wound treatment. To provide treatment evidence of cord blood stem cell on burn injury, CD34+ (hematopoietic lineage) and CD34? cells (mesenchymal stem cells lineage) were isolated and applied to full thickness skin wounds for evaluation of their effect.

Methods: Mononuclear cell layer was separated by Ficoll- Paque from human cord blood, followed by selection of CD34+, ? cells. Two full-thickness wounds were made on dorsal area of ICR mice with 8 mm punch. CD 34+, ? cell suspension was injected into the wound sites and wound healing rate was measured every 3 days with analysis of neo- vascularization. Immunohistochemical study for stem cell survival and TGF-?1 was performed.

Results: The wound healing rate of CD34? , CD34+ and control group was 34.73%, 9.24%, 5.132% on day 3 and 82.60%, 64.45% 76.20% on day 9 respectively. The CD34? group showed a significantly higher wound healing rate (p<0.01). The CD34? group revealed a relatively well organized epidermis and showed a remarkably increased neo-vascularization. Anti-human nuclei was detected on CD 34? group. TGF-?1 was stained all groups with no differences.

Conclusion: CD34? mononuclear cells group showed increased wound healing than the control and CD34+ mononuclear cells treated group. CD34? mononuclear cells enhanced epithelization and neo-vascularization by means of differentiation into endothelial cells, myofibroblast and so on. We can conclude that isolated CD34? mononuclear cells can be helpful for burn wound.
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1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Rah, Dong Kyun(나동균)
Lew, Dae Hyun(유대현)
Tark, Kwan Chul(탁관철)
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