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IgE-binding reactivity of peptide fragments of Bla g 1.02, a major German cockroach allergen

Authors
 Myung-hee Yi  ;  Kyoung Yong Jeong  ;  Chung-Ryul Kim  ;  Tai-Soon Yong 
Citation
 Asian Pacific Journal of Allergy and Immunology, Vol.27(2-3) : 121-129, 2009 
Journal Title
 Asian Pacific Journal of Allergy and Immunology 
ISSN
 0125-877X 
Issue Date
2009
MeSH
Allergens/genetics ; Allergens/immunology ; Allergens/metabolism* ; Animals ; Antigens, Plant ; Cloning, Molecular ; Cockroaches/immunology ; Epitope Mapping ; Humans ; Hypersensitivity/immunology* ; Immunodominant Epitopes/genetics ; Immunodominant Epitopes/immunology ; Immunodominant Epitopes/metabolism* ; Immunoglobulin E/blood ; Immunoglobulin E/immunology* ; Immunologic Tests ; Peptide Fragments/genetics ; Peptide Fragments/immunology ; Peptide Fragments/metabolism* ; Protein Binding
Abstract
Cockroaches cause allergic diseases and are closely linked with the development of asthma. Bla g 1 is one of the major allergen proteins produced by German cockroaches. It consists of tandem repeats of approximately 100 amino acids. The aim of the present study was to identify linear IgE-binding epitopes of Bla g 1.02. RT-PCR was used to clone a cDNA sequence encoding Bla g 1.02 (EF202179) which shared 98.6-99.8% identity with a previously reported Bla g 1.02 (AF072220). To investigate IgE binding regions, five separate but overlapping Bla g 1.02 peptide fragments (A: aa 1-111, B: aa 102-215, C: aa 206-299, D: aa 289-403, E: aa 394-491) were amplified and cloned. The full-length and five peptide fragments were overexpressed in Pichia pastoris and E. coli, respectively, and their IgE binding reactivities were measured by ELISA using 37 serum samples isolated from cockroach-sensitized patients. The sera of 24 patients (64.9%) recognized the full-length Bla g 1.02 recombinant protein. Among 19 selected serum samples, 11 sera (57.9%) reacted to peptide fragment A, 5 sera (31.3%) to B, 4 sera (21.1%) to C, 9 sera (47.4%) to D, and 10 sera (52.6%) to peptide fragment E. IgE-binding epitopes are found to be distributed to each tandem repeat of Bla g 1. The combination of peptide fragments A, D, and E may able to detect all Bla g 1-sensitized subjects. We suggest that these peptide fragments may be useful in allergy diagnosis and the design of novel immunotherapeutics.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
Yonsei Authors
Kim, Chung Ryul(김충렬)
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Yi, Myung Hee(이명희) ORCID logo https://orcid.org/0000-0001-9537-5726
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105042
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