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IgE-binding reactivity of peptide fragments of Bla g 1.02, a major German cockroach allergen

DC Field Value Language
dc.contributor.author김충렬-
dc.contributor.author용태순-
dc.contributor.author이명희-
dc.contributor.author정경용-
dc.date.accessioned2015-04-24T17:15:07Z-
dc.date.available2015-04-24T17:15:07Z-
dc.date.issued2009-
dc.identifier.issn0125-877X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/105042-
dc.description.abstractCockroaches cause allergic diseases and are closely linked with the development of asthma. Bla g 1 is one of the major allergen proteins produced by German cockroaches. It consists of tandem repeats of approximately 100 amino acids. The aim of the present study was to identify linear IgE-binding epitopes of Bla g 1.02. RT-PCR was used to clone a cDNA sequence encoding Bla g 1.02 (EF202179) which shared 98.6-99.8% identity with a previously reported Bla g 1.02 (AF072220). To investigate IgE binding regions, five separate but overlapping Bla g 1.02 peptide fragments (A: aa 1-111, B: aa 102-215, C: aa 206-299, D: aa 289-403, E: aa 394-491) were amplified and cloned. The full-length and five peptide fragments were overexpressed in Pichia pastoris and E. coli, respectively, and their IgE binding reactivities were measured by ELISA using 37 serum samples isolated from cockroach-sensitized patients. The sera of 24 patients (64.9%) recognized the full-length Bla g 1.02 recombinant protein. Among 19 selected serum samples, 11 sera (57.9%) reacted to peptide fragment A, 5 sera (31.3%) to B, 4 sera (21.1%) to C, 9 sera (47.4%) to D, and 10 sera (52.6%) to peptide fragment E. IgE-binding epitopes are found to be distributed to each tandem repeat of Bla g 1. The combination of peptide fragments A, D, and E may able to detect all Bla g 1-sensitized subjects. We suggest that these peptide fragments may be useful in allergy diagnosis and the design of novel immunotherapeutics.-
dc.description.statementOfResponsibilityopen-
dc.format.extent121~129-
dc.relation.isPartOfASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAllergens/genetics-
dc.subject.MESHAllergens/immunology-
dc.subject.MESHAllergens/metabolism*-
dc.subject.MESHAnimals-
dc.subject.MESHAntigens, Plant-
dc.subject.MESHCloning, Molecular-
dc.subject.MESHCockroaches/immunology-
dc.subject.MESHEpitope Mapping-
dc.subject.MESHHumans-
dc.subject.MESHHypersensitivity/immunology*-
dc.subject.MESHImmunodominant Epitopes/genetics-
dc.subject.MESHImmunodominant Epitopes/immunology-
dc.subject.MESHImmunodominant Epitopes/metabolism*-
dc.subject.MESHImmunoglobulin E/blood-
dc.subject.MESHImmunoglobulin E/immunology*-
dc.subject.MESHImmunologic Tests-
dc.subject.MESHPeptide Fragments/genetics-
dc.subject.MESHPeptide Fragments/immunology-
dc.subject.MESHPeptide Fragments/metabolism*-
dc.subject.MESHProtein Binding-
dc.titleIgE-binding reactivity of peptide fragments of Bla g 1.02, a major German cockroach allergen-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학)-
dc.contributor.googleauthorMyung-hee Yi-
dc.contributor.googleauthorKyoung Yong Jeong-
dc.contributor.googleauthorChung-Ryul Kim-
dc.contributor.googleauthorTai-Soon Yong-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01062-
dc.contributor.localIdA02424-
dc.contributor.localIdA02761-
dc.contributor.localIdA03572-
dc.relation.journalcodeJ03115-
dc.identifier.pmid19839498-
dc.contributor.alternativeNameKim, Chung Ryul-
dc.contributor.alternativeNameYong, Tai Soon-
dc.contributor.alternativeNameYi, Myung Hee-
dc.contributor.alternativeNameJeong, Kyoung Yong-
dc.contributor.affiliatedAuthorKim, Chung Ryul-
dc.contributor.affiliatedAuthorYong, Tai Soon-
dc.contributor.affiliatedAuthorYi, Myung Hee-
dc.contributor.affiliatedAuthorJeong, Kyoung Yong-
dc.citation.volume27-
dc.citation.number2-3-
dc.citation.startPage121-
dc.citation.endPage129-
dc.identifier.bibliographicCitationASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY, Vol.27(2-3) : 121-129, 2009-
dc.identifier.rimsid56251-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers

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