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Allergenicity of sigma and delta class glutathione S-transferases from the German cockroach.

 Kyoung Yong Jeong  ;  Kyoung-jin Jeong  ;  Myung-hee Yi  ;  Haeseok Lee  ;  Chein-Soo Hong  ;  Tai-Soon Yong 
 International Archives of Allergy and Immunology, Vol.148(1) : 59-64, 2009 
Journal Title
 International Archives of Allergy and Immunology 
Issue Date
Adolescent ; Adult ; Allergens/immunology* ; Allergens/metabolism ; Amino Acid Sequence ; Animals ; Child ; Cockroaches/enzymology ; Cockroaches/immunology* ; Cross Reactions ; Female ; Glutathione Transferase/genetics ; Glutathione Transferase/immunology* ; Glutathione Transferase/metabolism ; Humans ; Hypersensitivity/immunology* ; Immunoglobulin E/blood* ; Male ; Middle Aged ; Molecular Sequence Data ; Recombinant Proteins ; Sequence Alignment ; Young Adult
Allergen ; Bla g 5 ; Cockroach glutathione S-transferase ; IgE cross-reactivity
BACKGROUND: Cockroach glutathione S-transferases (GSTs) are known to elicit strong IgE responses. This study was undertaken to compare the IgE reactivity of German cockroach GSTs, Bla g 5 (sigma class) and delta class GST (BgGSTD1). METHODS: Full-length Bla g 5 and BgGSTD1 were cloned, and their recombinant proteins were expressed and purified. Their IgE reactivities and cross-reactivities were examined by ELISA using sera from cockroach-sensitized subjects. RESULTS: A predominant variant of Bla g 5 cDNA has amino acid substitutions at positions 10 (C to F) and 42 (N to K). BgGSTD1 has substitutions at positions 27 (E to N) and 207 (K to R). Sera from cockroach-sensitized patients showed 20.5% IgE reactivity to Bla g 5 and 17.9% IgE reactivity to BgGSTD1. However, inhibition studies using 1 serum sample with the highest IgE reactivity showed limited cross-reactivity. CONCLUSIONS: IgE-binding frequency to the cockroach GSTs was low, but the titer of IgE reactivity was strong in some sera. The inclusion of different classes of GSTs could be helpful for the delicate diagnosis and immunotherapy of cockroach allergy
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Yi, Myung Hee(이명희) ORCID logo https://orcid.org/0000-0001-9537-5726
Ree, Han Il(이한일)
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
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