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Giardia lamblia: immunogenicity and intracellular distribution of GHSP-115, a member of the Giardia head-stalk family of proteins.

Authors
 Sung Su Bae  ;  Juri Kim  ;  Tong-Soo Kim  ;  Tai-Soon Yong  ;  Soon-Jung Park 
Citation
 EXPERIMENTAL PARASITOLOGY, Vol.122(1) : 11-16, 2009 
Journal Title
 EXPERIMENTAL PARASITOLOGY 
ISSN
 0014-4894 
Issue Date
2009
MeSH
Amino Acid Sequence ; Animals ; Antibodies, Monoclonal/biosynthesis ; Antibodies, Monoclonal/immunology ; Blotting, Western ; Electrophoresis, Polyacrylamide Gel ; Female ; Giardia lamblia/chemistry ; Giardia lamblia/immunology* ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Protozoan Proteins/analysis ; Protozoan Proteins/chemistry ; Protozoan Proteins/immunology* ; Recombinant Proteins/analysis ; Recombinant Proteins/chemistry ; Recombinant Proteins/immunology
Keywords
Giardia lamblia ; Head-stalk protein ; Monoclonal antibodies
Abstract
Giardia lamblia, a protozoan causing diarrheal outbreaks, is one of the main pathogens monitored in developed countries. Immunoscreening of G. lamblia expression library using the monoclonal antibodies (mAb) against G. lamblia, identified a subset of antigenic proteins in this protozoan, which are proteins belonging to GHSP (Giardia head-stalk protein), GHSP115, GHSP138, and GHSP180. In order to map the epitope region of GHSP115, the corresponding open reading frame was dissected into three parts and expressed as recombinant proteins with histidine tags. Western blot analysis of these recombinant proteins with mAbs reacting with GHSP115 indicated that one-third of the C-terminus of GHSP115 showed immunoreactivity with the mAb. Intracellular location of GHSP115 was examined both in trophozoites and encysting cells of G. lamblia by an immunofluorescence assay, indicating that location of GHSP115 varies during encystation. These results suggest that GHSP115 is an abundant and antigenic protein, which is differentially localized during life cycle of G. lamblia.
Full Text
http://www.sciencedirect.com/science/article/pii/S001448940900006X
DOI
10.1016/j.exppara.2009.01.005
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105033
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