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Giardia lamblia: immunogenicity and intracellular distribution of GHSP-115, a member of the Giardia head-stalk family of proteins.

DC Field Value Language
dc.contributor.author용태순-
dc.contributor.author김주리-
dc.contributor.author박순정-
dc.date.accessioned2015-04-24T17:14:51Z-
dc.date.available2015-04-24T17:14:51Z-
dc.date.issued2009-
dc.identifier.issn0014-4894-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/105033-
dc.description.abstractGiardia lamblia, a protozoan causing diarrheal outbreaks, is one of the main pathogens monitored in developed countries. Immunoscreening of G. lamblia expression library using the monoclonal antibodies (mAb) against G. lamblia, identified a subset of antigenic proteins in this protozoan, which are proteins belonging to GHSP (Giardia head-stalk protein), GHSP115, GHSP138, and GHSP180. In order to map the epitope region of GHSP115, the corresponding open reading frame was dissected into three parts and expressed as recombinant proteins with histidine tags. Western blot analysis of these recombinant proteins with mAbs reacting with GHSP115 indicated that one-third of the C-terminus of GHSP115 showed immunoreactivity with the mAb. Intracellular location of GHSP115 was examined both in trophozoites and encysting cells of G. lamblia by an immunofluorescence assay, indicating that location of GHSP115 varies during encystation. These results suggest that GHSP115 is an abundant and antigenic protein, which is differentially localized during life cycle of G. lamblia.-
dc.description.statementOfResponsibilityopen-
dc.format.extent11~16-
dc.relation.isPartOfEXPERIMENTAL PARASITOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Monoclonal/biosynthesis-
dc.subject.MESHAntibodies, Monoclonal/immunology-
dc.subject.MESHBlotting, Western-
dc.subject.MESHElectrophoresis, Polyacrylamide Gel-
dc.subject.MESHFemale-
dc.subject.MESHGiardia lamblia/chemistry-
dc.subject.MESHGiardia lamblia/immunology*-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred BALB C-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHProtozoan Proteins/analysis-
dc.subject.MESHProtozoan Proteins/chemistry-
dc.subject.MESHProtozoan Proteins/immunology*-
dc.subject.MESHRecombinant Proteins/analysis-
dc.subject.MESHRecombinant Proteins/chemistry-
dc.subject.MESHRecombinant Proteins/immunology-
dc.titleGiardia lamblia: immunogenicity and intracellular distribution of GHSP-115, a member of the Giardia head-stalk family of proteins.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학)-
dc.contributor.googleauthorSung Su Bae-
dc.contributor.googleauthorJuri Kim-
dc.contributor.googleauthorTong-Soo Kim-
dc.contributor.googleauthorTai-Soon Yong-
dc.contributor.googleauthorSoon-Jung Park-
dc.identifier.doi10.1016/j.exppara.2009.01.005-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02424-
dc.contributor.localIdA00933-
dc.contributor.localIdA01545-
dc.relation.journalcodeJ00875-
dc.identifier.eissn1090-2449-
dc.identifier.pmid19545528-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S001448940900006X-
dc.subject.keywordGiardia lamblia-
dc.subject.keywordHead-stalk protein-
dc.subject.keywordMonoclonal antibodies-
dc.contributor.alternativeNameYong, Tai Soon-
dc.contributor.alternativeNameKim, Ju Ri-
dc.contributor.alternativeNamePark, Soon Jung-
dc.contributor.affiliatedAuthorYong, Tai Soon-
dc.contributor.affiliatedAuthorKim, Ju Ri-
dc.contributor.affiliatedAuthorPark, Soon Jung-
dc.citation.volume122-
dc.citation.number1-
dc.citation.startPage11-
dc.citation.endPage16-
dc.identifier.bibliographicCitationEXPERIMENTAL PARASITOLOGY, Vol.122(1) : 11-16, 2009-
dc.identifier.rimsid56246-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers

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