229 237

Cited 5 times in

Evaluation of alpha-tubulin as an antigenic and molecular probe to detect Giardia lamblia

Authors
 Juri Kim  ;  Myeong Heon Shin  ;  Kyoung-Ju Song  ;  Soon-Jung Park 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.47(3) : 287-291, 2009 
Journal Title
 KOREAN JOURNAL OF PARASITOLOGY 
ISSN
 0023-4001 
Issue Date
2009
MeSH
Animals ; Antigens, Protozoan/genetics* ; Antigens, Protozoan/immunology ; Base Sequence ; Giardia lamblia/genetics ; Giardia lamblia/immunology ; Giardia lamblia/isolation & purification* ; Giardiasis/diagnosis* ; Giardiasis/immunology ; Giardiasis/parasitology ; Humans ; Molecular Probes/genetics ; Molecular Probes/immunology ; Molecular Sequence Data ; Polymerase Chain Reaction/methods* ; Polymorphism, Restriction Fragment Length ; Protozoan Proteins/genetics* ; Protozoan Proteins/immunology ; Sequence Alignment ; Tubulin/genetics* ; Tubulin/immunology
Keywords
Giardia lamblia ; PCR-RFLP ; immunofluorescence assay ; α-tubulin
Abstract
The alpha/beta-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant alpha-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of alpha-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the alpha-tubulin gene from G. lamblia. PCR-RFLP analysis of this alpha-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that alpha-tubulin can be used as a molecular probe to detect G. lamblia
Files in This Item:
T200903447.pdf Download
DOI
10.3347/kjp.2009.47.3.287
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Song, Kyoung Ju(송경주)
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105029
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse