326 460

Cited 5 times in

Evaluation of alpha-tubulin as an antigenic and molecular probe to detect Giardia lamblia

DC Field Value Language
dc.contributor.author김주리-
dc.contributor.author박순정-
dc.contributor.author송경주-
dc.contributor.author신명헌-
dc.date.accessioned2015-04-24T17:14:43Z-
dc.date.available2015-04-24T17:14:43Z-
dc.date.issued2009-
dc.identifier.issn0023-4001-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/105029-
dc.description.abstractThe alpha/beta-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant alpha-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of alpha-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the alpha-tubulin gene from G. lamblia. PCR-RFLP analysis of this alpha-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that alpha-tubulin can be used as a molecular probe to detect G. lamblia-
dc.description.statementOfResponsibilityopen-
dc.format.extent287~291-
dc.relation.isPartOfKOREAN JOURNAL OF PARASITOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAntigens, Protozoan/genetics*-
dc.subject.MESHAntigens, Protozoan/immunology-
dc.subject.MESHBase Sequence-
dc.subject.MESHGiardia lamblia/genetics-
dc.subject.MESHGiardia lamblia/immunology-
dc.subject.MESHGiardia lamblia/isolation & purification*-
dc.subject.MESHGiardiasis/diagnosis*-
dc.subject.MESHGiardiasis/immunology-
dc.subject.MESHGiardiasis/parasitology-
dc.subject.MESHHumans-
dc.subject.MESHMolecular Probes/genetics-
dc.subject.MESHMolecular Probes/immunology-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPolymerase Chain Reaction/methods*-
dc.subject.MESHPolymorphism, Restriction Fragment Length-
dc.subject.MESHProtozoan Proteins/genetics*-
dc.subject.MESHProtozoan Proteins/immunology-
dc.subject.MESHSequence Alignment-
dc.subject.MESHTubulin/genetics*-
dc.subject.MESHTubulin/immunology-
dc.titleEvaluation of alpha-tubulin as an antigenic and molecular probe to detect Giardia lamblia-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학)-
dc.contributor.googleauthorJuri Kim-
dc.contributor.googleauthorMyeong Heon Shin-
dc.contributor.googleauthorKyoung-Ju Song-
dc.contributor.googleauthorSoon-Jung Park-
dc.identifier.doi10.3347/kjp.2009.47.3.287-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00933-
dc.contributor.localIdA01545-
dc.contributor.localIdA02012-
dc.contributor.localIdA02099-
dc.relation.journalcodeJ01957-
dc.identifier.eissn1738-0006-
dc.identifier.pmid19724704-
dc.subject.keywordGiardia lamblia-
dc.subject.keywordPCR-RFLP-
dc.subject.keywordimmunofluorescence assay-
dc.subject.keywordα-tubulin-
dc.contributor.alternativeNameKim, Ju Ri-
dc.contributor.alternativeNamePark, Soon Jung-
dc.contributor.alternativeNameSong, Kyoung Ju-
dc.contributor.alternativeNameShin, Myeong Heon-
dc.contributor.affiliatedAuthorKim, Ju Ri-
dc.contributor.affiliatedAuthorPark, Soon Jung-
dc.contributor.affiliatedAuthorSong, Kyoung Ju-
dc.contributor.affiliatedAuthorShin, Myeong Heon-
dc.citation.volume47-
dc.citation.number3-
dc.citation.startPage287-
dc.citation.endPage291-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF PARASITOLOGY, Vol.47(3) : 287-291, 2009-
dc.identifier.rimsid56242-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.