Cited 7 times in
Evaluation of alpha-tubulin as an antigenic and molecular probe to detect Giardia lamblia
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김주리 | - |
dc.contributor.author | 박순정 | - |
dc.contributor.author | 송경주 | - |
dc.contributor.author | 신명헌 | - |
dc.date.accessioned | 2015-04-24T17:14:43Z | - |
dc.date.available | 2015-04-24T17:14:43Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/105029 | - |
dc.description.abstract | The alpha/beta-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant alpha-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of alpha-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the alpha-tubulin gene from G. lamblia. PCR-RFLP analysis of this alpha-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that alpha-tubulin can be used as a molecular probe to detect G. lamblia | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 287~291 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PARASITOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, Protozoan/genetics* | - |
dc.subject.MESH | Antigens, Protozoan/immunology | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Giardia lamblia/genetics | - |
dc.subject.MESH | Giardia lamblia/immunology | - |
dc.subject.MESH | Giardia lamblia/isolation & purification* | - |
dc.subject.MESH | Giardiasis/diagnosis* | - |
dc.subject.MESH | Giardiasis/immunology | - |
dc.subject.MESH | Giardiasis/parasitology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Molecular Probes/genetics | - |
dc.subject.MESH | Molecular Probes/immunology | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Polymerase Chain Reaction/methods* | - |
dc.subject.MESH | Polymorphism, Restriction Fragment Length | - |
dc.subject.MESH | Protozoan Proteins/genetics* | - |
dc.subject.MESH | Protozoan Proteins/immunology | - |
dc.subject.MESH | Sequence Alignment | - |
dc.subject.MESH | Tubulin/genetics* | - |
dc.subject.MESH | Tubulin/immunology | - |
dc.title | Evaluation of alpha-tubulin as an antigenic and molecular probe to detect Giardia lamblia | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Juri Kim | - |
dc.contributor.googleauthor | Myeong Heon Shin | - |
dc.contributor.googleauthor | Kyoung-Ju Song | - |
dc.contributor.googleauthor | Soon-Jung Park | - |
dc.identifier.doi | 10.3347/kjp.2009.47.3.287 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00933 | - |
dc.contributor.localId | A01545 | - |
dc.contributor.localId | A02012 | - |
dc.contributor.localId | A02099 | - |
dc.relation.journalcode | J01957 | - |
dc.identifier.eissn | 1738-0006 | - |
dc.identifier.pmid | 19724704 | - |
dc.subject.keyword | Giardia lamblia | - |
dc.subject.keyword | PCR-RFLP | - |
dc.subject.keyword | immunofluorescence assay | - |
dc.subject.keyword | α-tubulin | - |
dc.contributor.alternativeName | Kim, Ju Ri | - |
dc.contributor.alternativeName | Park, Soon Jung | - |
dc.contributor.alternativeName | Song, Kyoung Ju | - |
dc.contributor.alternativeName | Shin, Myeong Heon | - |
dc.contributor.affiliatedAuthor | Kim, Ju Ri | - |
dc.contributor.affiliatedAuthor | Park, Soon Jung | - |
dc.contributor.affiliatedAuthor | Song, Kyoung Ju | - |
dc.contributor.affiliatedAuthor | Shin, Myeong Heon | - |
dc.citation.volume | 47 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 287 | - |
dc.citation.endPage | 291 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PARASITOLOGY, Vol.47(3) : 287-291, 2009 | - |
dc.identifier.rimsid | 56242 | - |
dc.type.rims | ART | - |
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