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IgE Binding Reactivity of Peptide Fragments of Bla g 4, a Major German Cockroach Allergen

Authors
 Kwang Hyun Shin  ;  Kyoung Yong Jeong  ;  Chein-Soo Hong  ;  Tai-Soon Yong 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.47(1) : 31-36, 2009 
Journal Title
 KOREAN JOURNAL OF PARASITOLOGY 
ISSN
 0023-4001 
Issue Date
2009
MeSH
Adolescent ; Adult ; Allergens/chemistry ; Allergens/genetics ; Allergens/immunology* ; Amino Acid Sequence ; Animals ; Antigens, Plant ; Child ; Cockroaches/immunology* ; Epitope Mapping* ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Female ; Humans ; Hypersensitivity/immunology* ; Immunoglobulin E/immunology* ; Insect Proteins/chemistry ; Insect Proteins/genetics ; Insect Proteins/immunology* ; Male ; Middle Aged ; Molecular Sequence Data ; Sequence Alignment ; Young Adult
Keywords
Blattella germanica ; Allergen ; B-cell epitope ; IgE ; lipocalin
Abstract
Cockroaches have been recognized as a major cause of asthma. Bla g 4 is one of the most important German cockroach allergens. The aim of this study is to investigate IgE reactivity to the recombinant Bla g 4 (rBla g 4) in the sera of allergic patients and identify linear IgE binding epitope. For protein expression, full-length Bla g 4 (EF202172) was divided into 5 overlapping peptide fragments (E1: aa 1-100, E2: aa 34-77, E3: aa 74-117, E4: aa 114-156, and E5: aa 153-182). The full-length and 5 peptide fragments of Bla g 4 was generated by PCR and over-expressed in E. coli BL21 (DE3). The IgE binding reactivities of the full-length and peptide fragments were measured by ELISA using 32 serum samples of cockroach allergy. The sera of 8 patients (25%) reacted with rBla g 4. Four sera (100%) showed IgE-binding reactivity to full-length and peptide fragment 4, and 2 sera (50%) reacted with peptide fragment 2. One (20%) serum reacted with peptide fragment 3. The results of ELISA using overlapping recombinant fragments indicated that the epitope region was located at amino acid sequences 34-73 and 78-113, and major IgE epitope of Bla g 4 was located at amino acid sequences 118-152 of C-terminal. B-cell epitope analysis of German cockroach allergen Bla g 4 could contribute to the strategic development of more specific and potentially efficacious immunotherapy
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104805
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