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IgE Binding Reactivity of Peptide Fragments of Bla g 4, a Major German Cockroach Allergen
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 용태순 | - |
dc.contributor.author | 정경용 | - |
dc.contributor.author | 홍천수 | - |
dc.date.accessioned | 2015-04-24T17:07:27Z | - |
dc.date.available | 2015-04-24T17:07:27Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104805 | - |
dc.description.abstract | Cockroaches have been recognized as a major cause of asthma. Bla g 4 is one of the most important German cockroach allergens. The aim of this study is to investigate IgE reactivity to the recombinant Bla g 4 (rBla g 4) in the sera of allergic patients and identify linear IgE binding epitope. For protein expression, full-length Bla g 4 (EF202172) was divided into 5 overlapping peptide fragments (E1: aa 1-100, E2: aa 34-77, E3: aa 74-117, E4: aa 114-156, and E5: aa 153-182). The full-length and 5 peptide fragments of Bla g 4 was generated by PCR and over-expressed in E. coli BL21 (DE3). The IgE binding reactivities of the full-length and peptide fragments were measured by ELISA using 32 serum samples of cockroach allergy. The sera of 8 patients (25%) reacted with rBla g 4. Four sera (100%) showed IgE-binding reactivity to full-length and peptide fragment 4, and 2 sera (50%) reacted with peptide fragment 2. One (20%) serum reacted with peptide fragment 3. The results of ELISA using overlapping recombinant fragments indicated that the epitope region was located at amino acid sequences 34-73 and 78-113, and major IgE epitope of Bla g 4 was located at amino acid sequences 118-152 of C-terminal. B-cell epitope analysis of German cockroach allergen Bla g 4 could contribute to the strategic development of more specific and potentially efficacious immunotherapy | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 31~36 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PARASITOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adolescent | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Allergens/chemistry | - |
dc.subject.MESH | Allergens/genetics | - |
dc.subject.MESH | Allergens/immunology* | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, Plant | - |
dc.subject.MESH | Child | - |
dc.subject.MESH | Cockroaches/immunology* | - |
dc.subject.MESH | Epitope Mapping* | - |
dc.subject.MESH | Escherichia coli/genetics | - |
dc.subject.MESH | Escherichia coli/metabolism | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypersensitivity/immunology* | - |
dc.subject.MESH | Immunoglobulin E/immunology* | - |
dc.subject.MESH | Insect Proteins/chemistry | - |
dc.subject.MESH | Insect Proteins/genetics | - |
dc.subject.MESH | Insect Proteins/immunology* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Sequence Alignment | - |
dc.subject.MESH | Young Adult | - |
dc.title | IgE Binding Reactivity of Peptide Fragments of Bla g 4, a Major German Cockroach Allergen | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Kwang Hyun Shin | - |
dc.contributor.googleauthor | Kyoung Yong Jeong | - |
dc.contributor.googleauthor | Chein-Soo Hong | - |
dc.contributor.googleauthor | Tai-Soon Yong | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02424 | - |
dc.contributor.localId | A03572 | - |
dc.contributor.localId | A04448-1 | - |
dc.relation.journalcode | J01957 | - |
dc.identifier.eissn | 1738-0006 | - |
dc.identifier.pmid | 19290089 | - |
dc.subject.keyword | Blattella germanica | - |
dc.subject.keyword | Allergen | - |
dc.subject.keyword | B-cell epitope | - |
dc.subject.keyword | IgE | - |
dc.subject.keyword | lipocalin | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.alternativeName | Jeong, Kyoung Yong | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Jeong, Kyoung Yong | - |
dc.contributor.affiliatedAuthor | Hong, Chein Soo | - |
dc.citation.volume | 47 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 31 | - |
dc.citation.endPage | 36 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PARASITOLOGY, Vol.47(1) : 31-36, 2009 | - |
dc.identifier.rimsid | 40802 | - |
dc.type.rims | ART | - |
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