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Diagnosis and Species Identification of Mycobacterial Infections by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Sterile Body Fluids

 Cheong Ho Cho  ;  Sang Hoon Han  ;  Bum Sik Chin  ;  Suk Hoon Choi  ;  Han Sung Lee  ;  Chang Oh Kim  ;  Myung Soo Kim  ;  Jun Yong Choi  ;  Young Goo Song  ;  June Myung Kim 
 Korean Journal of Internal Medicine, Vol.24(2) : 135-138, 2009 
Journal Title
 Korean Journal of Internal Medicine 
Issue Date
BACKGROUND/AIMS: The development of effective, accurate, and rapid diagnostic methods for Mycobacterium infection and mycobacterial species identification is required. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is an easy, rapid and inexpensive technique for identifying Mycobacterium spp. METHODS: We performed PCR-RFLP to detect and identify Mycobacterium spp. from 10 sterile body fluids, including ascites, cerebrospinal fluid, pleural fluid, synovial fluid, and peritoneal dialysis fluid. Clinical samples were collected from patients with diagnoses of definite, probable or suspected mycobacterial infection. The conserved RNA polymerase genes of Mycobacterium spp. were amplified by PCR. RESULTS: The amplified 360-bp region of rpoB was digested with the restriction enzyme MspI or HaeIII. The PCRRFLP results for the clinical samples were identical to those for M. tuberculosis, M. fortuitum, M. intracellulare, and M. avium. In addition, the results of the PCR-RFLP were identical to those obtained by DNA sequencing. CONCLUSIONS: PCR-RFLP analysis of sterile body fluids may be a useful method for the diagnosis of mycobacterial infections and for the differentiation of mycobacterial species
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1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
김명수(Kim, Myoung Soo) ORCID logo https://orcid.org/0000-0002-8975-8381
김준명(Kim, June Myung)
김창오(Kim, Chang Oh) ORCID logo https://orcid.org/0000-0002-0773-5443
송영구(Song, Young Goo) ORCID logo https://orcid.org/0000-0002-0733-4156
이한성(Lee, Han Sung)
조정호(Cho, Cheong Ho)
진범식(Chin, Bum Sik)
최석훈(Choi, Suk Hoon)
최준용(Choi, Jun Yong) ORCID logo https://orcid.org/0000-0002-2775-3315
한상훈(Han, Sang Hoon) ORCID logo https://orcid.org/0000-0002-4278-5198
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