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Diagnosis and Species Identification of Mycobacterial Infections by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Sterile Body Fluids

 Cheong Ho Cho  ;  Sang Hoon Han  ;  Bum Sik Chin  ;  Suk Hoon Choi  ;  Han Sung Lee  ;  Chang Oh Kim  ;  Myung Soo Kim  ;  Jun Yong Choi  ;  Young Goo Song  ;  June Myung Kim 
 KOREAN JOURNAL OF INTERNAL MEDICINE, Vol.24(2) : 135-138, 2009 
Journal Title
Issue Date
Amplified Fragment Length Polymorphism Analysis* ; Bacterial Proteins/genetics* ; Bacterial Typing Techniques* ; Body Fluids/microbiology* ; DNA, Bacterial/analysis* ; DNA-Directed RNA Polymerases/genetics* ; Humans ; Mycobacterium/classification* ; Mycobacterium/genetics ; Mycobacterium Infections/diagnosis* ; Mycobacterium Infections/microbiology ; Polymorphism, Restriction Fragment Length*
Mycobacterium infections ; Polymerase chain reaction ; Restriction fragment length polymorphism
BACKGROUND/AIMS: The development of effective, accurate, and rapid diagnostic methods for Mycobacterium infection and mycobacterial species identification is required. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is an easy, rapid and inexpensive technique for identifying Mycobacterium spp. METHODS: We performed PCR-RFLP to detect and identify Mycobacterium spp. from 10 sterile body fluids, including ascites, cerebrospinal fluid, pleural fluid, synovial fluid, and peritoneal dialysis fluid. Clinical samples were collected from patients with diagnoses of definite, probable or suspected mycobacterial infection. The conserved RNA polymerase genes of Mycobacterium spp. were amplified by PCR. RESULTS: The amplified 360-bp region of rpoB was digested with the restriction enzyme MspI or HaeIII. The PCRRFLP results for the clinical samples were identical to those for M. tuberculosis, M. fortuitum, M. intracellulare, and M. avium. In addition, the results of the PCR-RFLP were identical to those obtained by DNA sequencing. CONCLUSIONS: PCR-RFLP analysis of sterile body fluids may be a useful method for the diagnosis of mycobacterial infections and for the differentiation of mycobacterial species
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1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Soo(김명수) ORCID logo https://orcid.org/0000-0002-8975-8381
Kim, June Myung(김준명)
Kim, Chang Oh(김창오) ORCID logo https://orcid.org/0000-0002-0773-5443
Song, Young Goo(송영구) ORCID logo https://orcid.org/0000-0002-0733-4156
Lee, Han Sung(이한성)
Cho, Cheong Ho(조정호)
Chin, Bum Sik(진범식)
Choi, Suk Hoon(최석훈)
Choi, Jun Yong(최준용) ORCID logo https://orcid.org/0000-0002-2775-3315
Han, Sang Hoon(한상훈) ORCID logo https://orcid.org/0000-0002-4278-5198
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