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Diagnosis and Species Identification of Mycobacterial Infections by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Sterile Body Fluids

DC FieldValueLanguage
dc.contributor.author김명수-
dc.contributor.author김준명-
dc.contributor.author김창오-
dc.contributor.author송영구-
dc.contributor.author이한성-
dc.contributor.author조정호-
dc.contributor.author진범식-
dc.contributor.author최석훈-
dc.contributor.author최준용-
dc.contributor.author한상훈-
dc.date.accessioned2015-04-24T16:54:43Z-
dc.date.available2015-04-24T16:54:43Z-
dc.date.issued2009-
dc.identifier.issn1226-3303-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/104405-
dc.description.abstractBACKGROUND/AIMS: The development of effective, accurate, and rapid diagnostic methods for Mycobacterium infection and mycobacterial species identification is required. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is an easy, rapid and inexpensive technique for identifying Mycobacterium spp. METHODS: We performed PCR-RFLP to detect and identify Mycobacterium spp. from 10 sterile body fluids, including ascites, cerebrospinal fluid, pleural fluid, synovial fluid, and peritoneal dialysis fluid. Clinical samples were collected from patients with diagnoses of definite, probable or suspected mycobacterial infection. The conserved RNA polymerase genes of Mycobacterium spp. were amplified by PCR. RESULTS: The amplified 360-bp region of rpoB was digested with the restriction enzyme MspI or HaeIII. The PCRRFLP results for the clinical samples were identical to those for M. tuberculosis, M. fortuitum, M. intracellulare, and M. avium. In addition, the results of the PCR-RFLP were identical to those obtained by DNA sequencing. CONCLUSIONS: PCR-RFLP analysis of sterile body fluids may be a useful method for the diagnosis of mycobacterial infections and for the differentiation of mycobacterial species-
dc.description.statementOfResponsibilityopen-
dc.format.extent135~138-
dc.relation.isPartOfKOREAN JOURNAL OF INTERNAL MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmplified Fragment Length Polymorphism Analysis*-
dc.subject.MESHBacterial Proteins/genetics*-
dc.subject.MESHBacterial Typing Techniques*-
dc.subject.MESHBody Fluids/microbiology*-
dc.subject.MESHDNA, Bacterial/analysis*-
dc.subject.MESHDNA-Directed RNA Polymerases/genetics*-
dc.subject.MESHHumans-
dc.subject.MESHMycobacterium/classification*-
dc.subject.MESHMycobacterium/genetics-
dc.subject.MESHMycobacterium Infections/diagnosis*-
dc.subject.MESHMycobacterium Infections/microbiology-
dc.subject.MESHPolymorphism, Restriction Fragment Length*-
dc.titleDiagnosis and Species Identification of Mycobacterial Infections by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Sterile Body Fluids-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorCheong Ho Cho-
dc.contributor.googleauthorSang Hoon Han-
dc.contributor.googleauthorBum Sik Chin-
dc.contributor.googleauthorSuk Hoon Choi-
dc.contributor.googleauthorHan Sung Lee-
dc.contributor.googleauthorChang Oh Kim-
dc.contributor.googleauthorMyung Soo Kim-
dc.contributor.googleauthorJun Yong Choi-
dc.contributor.googleauthorYoung Goo Song-
dc.contributor.googleauthorJune Myung Kim-
dc.identifier.doi10.3904/kjim.2009.24.2.135-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00953-
dc.contributor.localIdA01044-
dc.contributor.localIdA02037-
dc.contributor.localIdA03277-
dc.contributor.localIdA03905-
dc.contributor.localIdA03981-
dc.contributor.localIdA04071-
dc.contributor.localIdA04191-
dc.contributor.localIdA04286-
dc.contributor.localIdA00424-
dc.relation.journalcodeJ02883-
dc.identifier.eissn2005-6648-
dc.identifier.pmid19543492-
dc.subject.keywordMycobacterium infections-
dc.subject.keywordPolymerase chain reaction-
dc.subject.keywordRestriction fragment length polymorphism-
dc.contributor.alternativeNameKim, Myoung Soo-
dc.contributor.alternativeNameKim, June Myung-
dc.contributor.alternativeNameKim, Chang Oh-
dc.contributor.alternativeNameSong, Young Goo-
dc.contributor.alternativeNameLee, Han Sung-
dc.contributor.alternativeNameCho, Cheong Ho-
dc.contributor.alternativeNameChin, Bum Sik-
dc.contributor.alternativeNameChoi, Suk Hoon-
dc.contributor.alternativeNameChoi, Jun Yong-
dc.contributor.alternativeNameHan, Sang Hoon-
dc.contributor.affiliatedAuthorKim, June Myung-
dc.contributor.affiliatedAuthorKim, Chang Oh-
dc.contributor.affiliatedAuthorSong, Young Goo-
dc.contributor.affiliatedAuthorLee, Han Sung-
dc.contributor.affiliatedAuthorCho, Cheong Ho-
dc.contributor.affiliatedAuthorChin, Bum Sik-
dc.contributor.affiliatedAuthorChoi, Suk Hoon-
dc.contributor.affiliatedAuthorChoi, Jun Yong-
dc.contributor.affiliatedAuthorHan, Sang Hoon-
dc.contributor.affiliatedAuthorKim, Myoung Soo-
dc.citation.volume24-
dc.citation.number2-
dc.citation.startPage135-
dc.citation.endPage138-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF INTERNAL MEDICINE, Vol.24(2) : 135-138, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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