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Bovine colostrum inhibits nuclear factor kappaB-mediated proinflammatory cytokine expression in intestinal epithelial cells

 Won Ho Kim  ;  Min Ji An  ;  Tae Il Kim  ;  Eun Soo Kim  ;  Song Yi Han  ;  Chang Mo Moon  ;  Jae Jun Park  ;  Seung Won Kim  ;  Jae Hee Cheon 
 NUTRITION RESEARCH, Vol.29(4) : 275-280, 2009 
Journal Title
Issue Date
Active Transport, Cell Nucleus ; Animals ; Anti-Inflammatory Agents/pharmacology* ; Cattle ; Cell Adhesion Molecules/antagonists & inhibitors ; Cell Survival/drug effects ; Colostrum* ; Cyclooxygenase 2/metabolism ; Cyclooxygenase 2 Inhibitors/pharmacology ; Epithelial Cells/drug effects* ; Female ; Gene Expression/drug effects ; HT29 Cells ; Humans ; I-kappa B Proteins/antagonists & inhibitors ; Inflammation/genetics ; Inflammation/metabolism* ; Interleukin-1beta/antagonists & inhibitors* ; Interleukin-8/antagonists & inhibitors* ; Intestines/cytology ; Intestines/drug effects ; NF-KappaB Inhibitor alpha ; NF-kappa B/antagonists & inhibitors* ; RNA, Messenger/metabolism ; Signal Transduction/drug effects ; Transcription Factor RelA/antagonists & inhibitors
Bovine ; Colostrum ; NF-κB ; Intestinal ; Inflammation ; HT-29 cells
Colostrum, a nutrient-rich fluid produced by female mammals immediately after giving birth, is loaded with several immune, growth, and tissue repair factors. However, it remains unknown whether bovine colostrum has anti-inflammatory effects on intestinal epithelial cells (IEC). In this study, we aimed to investigate the anti-inflammatory effects of colostrum on IEC and to elucidate its molecular mechanisms. Human colon cancer HT-29 cells were stimulated with interleukin (IL)-1beta with or without bovine colostrum. The effects of colostrum on nuclear factor kappaB (NF-kappaB) signaling in HT-29 cells were examined using real-time reverse transcriptase-polymerase chain reaction detect IL-8 and intracellar adhesion molecule-1 mRNA expression using a NF-kappaB-dependent reporter gene assay and an electrophoretic mobility shift assay. Furthermore, we assessed the expression levels of inhibitor protein of NF-kappaB-alpha, cyclooxygenase-2, and p65 proteins by Western blotting. Bovine colostrum significantly inhibited IL-1beta-induced IL-8 and intracellar adhesion molecule-1 mRNA expression. Moreover, it suppressed IL-1beta-induced NF-kappaB activation, including NF-kappaB dependent reporter gene expression in a dose-dependent manner. Finally, Western blotting revealed that colostrum decreased the cyclooxygenase-2 protein expression level, inhibited inhibitor protein of NF-kappaB-alpha degradation, and blocked translocation of p65 into the nucleus. These data demonstrated that bovine colostrum might protect against IEC inflammation by inhibiting the NF-kappaB pathway, suggesting colostrum has a therapeutic potential for intestinal inflammation
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kim, Seung Won(김승원) ORCID logo https://orcid.org/0000-0002-1692-1192
Kim, Won Ho(김원호) ORCID logo https://orcid.org/0000-0002-5682-9972
Kim, Eun Soo(김은수)
Kim, Tae Il(김태일) ORCID logo https://orcid.org/0000-0003-4807-890X
Moon, Chang Mo(문창모)
Park, Jae Jun(박재준)
Cheon, Jae Hee(천재희) ORCID logo https://orcid.org/0000-0002-2282-8904
Han, Song Yi(한송이)
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