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Bovine colostrum inhibits nuclear factor kappaB-mediated proinflammatory cytokine expression in intestinal epithelial cells

Authors
 Won Ho Kim  ;  Min Ji An  ;  Tae Il Kim  ;  Eun Soo Kim  ;  Song Yi Han  ;  Chang Mo Moon  ;  Jae Jun Park  ;  Seung Won Kim  ;  Jae Hee Cheon 
Citation
 Nutrition Research, Vol.29(4), 2009 
Journal Title
 Nutrition Research 
ISSN
 0271-5317 
Issue Date
2009
Abstract
Colostrum, a nutrient-rich fluid produced by female mammals immediately after giving birth, is loaded with several immune, growth, and tissue repair factors. However, it remains unknown whether bovine colostrum has anti-inflammatory effects on intestinal epithelial cells (IEC). In this study, we aimed to investigate the anti-inflammatory effects of colostrum on IEC and to elucidate its molecular mechanisms. Human colon cancer HT-29 cells were stimulated with interleukin (IL)-1beta with or without bovine colostrum. The effects of colostrum on nuclear factor kappaB (NF-kappaB) signaling in HT-29 cells were examined using real-time reverse transcriptase-polymerase chain reaction detect IL-8 and intracellar adhesion molecule-1 mRNA expression using a NF-kappaB-dependent reporter gene assay and an electrophoretic mobility shift assay. Furthermore, we assessed the expression levels of inhibitor protein of NF-kappaB-alpha, cyclooxygenase-2, and p65 proteins by Western blotting. Bovine colostrum significantly inhibited IL-1beta-induced IL-8 and intracellar adhesion molecule-1 mRNA expression. Moreover, it suppressed IL-1beta-induced NF-kappaB activation, including NF-kappaB dependent reporter gene expression in a dose-dependent manner. Finally, Western blotting revealed that colostrum decreased the cyclooxygenase-2 protein expression level, inhibited inhibitor protein of NF-kappaB-alpha degradation, and blocked translocation of p65 into the nucleus. These data demonstrated that bovine colostrum might protect against IEC inflammation by inhibiting the NF-kappaB pathway, suggesting colostrum has a therapeutic potential for intestinal inflammation
Full Text
http://www.sciencedirect.com/science/article/pii/S0271531709000554
DOI
10.1016/j.nutres.2009.03.011
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
김승원(Kim, Seung Won) ORCID logo https://orcid.org/0000-0002-1692-1192
김원호(Kim, Won Ho) ORCID logo https://orcid.org/0000-0002-5682-9972
김은수(Kim, Eun Soo)
김태일(Kim, Tae Il) ORCID logo https://orcid.org/0000-0003-4807-890X
문창모(Moon, Chang Mo)
박재준(Park, Jae Jun)
천재희(Cheon, Jae Hee) ORCID logo https://orcid.org/0000-0002-2282-8904
한송이(Han, Song Yi)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103898
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