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Bovine colostrum inhibits nuclear factor kappaB-mediated proinflammatory cytokine expression in intestinal epithelial cells

DC Field Value Language
dc.contributor.author천재희-
dc.contributor.author한송이-
dc.contributor.author김원호-
dc.contributor.author김은수-
dc.contributor.author김태일-
dc.contributor.author문창모-
dc.contributor.author박재준-
dc.contributor.author김승원-
dc.date.accessioned2015-04-24T16:38:33Z-
dc.date.available2015-04-24T16:38:33Z-
dc.date.issued2009-
dc.identifier.issn0271-5317-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/103898-
dc.description.abstractColostrum, a nutrient-rich fluid produced by female mammals immediately after giving birth, is loaded with several immune, growth, and tissue repair factors. However, it remains unknown whether bovine colostrum has anti-inflammatory effects on intestinal epithelial cells (IEC). In this study, we aimed to investigate the anti-inflammatory effects of colostrum on IEC and to elucidate its molecular mechanisms. Human colon cancer HT-29 cells were stimulated with interleukin (IL)-1beta with or without bovine colostrum. The effects of colostrum on nuclear factor kappaB (NF-kappaB) signaling in HT-29 cells were examined using real-time reverse transcriptase-polymerase chain reaction detect IL-8 and intracellar adhesion molecule-1 mRNA expression using a NF-kappaB-dependent reporter gene assay and an electrophoretic mobility shift assay. Furthermore, we assessed the expression levels of inhibitor protein of NF-kappaB-alpha, cyclooxygenase-2, and p65 proteins by Western blotting. Bovine colostrum significantly inhibited IL-1beta-induced IL-8 and intracellar adhesion molecule-1 mRNA expression. Moreover, it suppressed IL-1beta-induced NF-kappaB activation, including NF-kappaB dependent reporter gene expression in a dose-dependent manner. Finally, Western blotting revealed that colostrum decreased the cyclooxygenase-2 protein expression level, inhibited inhibitor protein of NF-kappaB-alpha degradation, and blocked translocation of p65 into the nucleus. These data demonstrated that bovine colostrum might protect against IEC inflammation by inhibiting the NF-kappaB pathway, suggesting colostrum has a therapeutic potential for intestinal inflammation-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfNUTRITION RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHActive Transport, Cell Nucleus-
dc.subject.MESHAnimals-
dc.subject.MESHAnti-Inflammatory Agents/pharmacology*-
dc.subject.MESHCattle-
dc.subject.MESHCell Adhesion Molecules/antagonists & inhibitors-
dc.subject.MESHCell Survival/drug effects-
dc.subject.MESHColostrum*-
dc.subject.MESHCyclooxygenase 2/metabolism-
dc.subject.MESHCyclooxygenase 2 Inhibitors/pharmacology-
dc.subject.MESHEpithelial Cells/drug effects*-
dc.subject.MESHFemale-
dc.subject.MESHGene Expression/drug effects-
dc.subject.MESHHT29 Cells-
dc.subject.MESHHumans-
dc.subject.MESHI-kappa B Proteins/antagonists & inhibitors-
dc.subject.MESHInflammation/genetics-
dc.subject.MESHInflammation/metabolism*-
dc.subject.MESHInterleukin-1beta/antagonists & inhibitors*-
dc.subject.MESHInterleukin-8/antagonists & inhibitors*-
dc.subject.MESHIntestines/cytology-
dc.subject.MESHIntestines/drug effects-
dc.subject.MESHNF-KappaB Inhibitor alpha-
dc.subject.MESHNF-kappa B/antagonists & inhibitors*-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHSignal Transduction/drug effects-
dc.subject.MESHTranscription Factor RelA/antagonists & inhibitors-
dc.titleBovine colostrum inhibits nuclear factor kappaB-mediated proinflammatory cytokine expression in intestinal epithelial cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorWon Ho Kim-
dc.contributor.googleauthorMin Ji An-
dc.contributor.googleauthorTae Il Kim-
dc.contributor.googleauthorEun Soo Kim-
dc.contributor.googleauthorSong Yi Han-
dc.contributor.googleauthorChang Mo Moon-
dc.contributor.googleauthorJae Jun Park-
dc.contributor.googleauthorSeung Won Kim-
dc.contributor.googleauthorJae Hee Cheon-
dc.identifier.doi10.1016/j.nutres.2009.03.011-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00774-
dc.contributor.localIdA01079-
dc.contributor.localIdA01390-
dc.contributor.localIdA01636-
dc.contributor.localIdA04291-
dc.contributor.localIdA00804-
dc.contributor.localIdA04030-
dc.relation.journalcodeJ02401-
dc.identifier.eissn1879-0739-
dc.identifier.pmid19410980-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0271531709000554-
dc.subject.keywordBovine-
dc.subject.keywordColostrum-
dc.subject.keywordNF-κB-
dc.subject.keywordIntestinal-
dc.subject.keywordInflammation-
dc.subject.keywordHT-29 cells-
dc.contributor.alternativeNameCheon, Jae Hee-
dc.contributor.alternativeNameHan, Song Yi-
dc.contributor.alternativeNameKim, Won Ho-
dc.contributor.alternativeNameKim, Eun Soo-
dc.contributor.alternativeNameKim, Tae Il-
dc.contributor.alternativeNameMoon, Chang Mo-
dc.contributor.alternativeNamePark, Jae Jun-
dc.contributor.affiliatedAuthorKim, Won Ho-
dc.contributor.affiliatedAuthorKim, Tae Il-
dc.contributor.affiliatedAuthorMoon, Chang Mo-
dc.contributor.affiliatedAuthorPark, Jae Jun-
dc.contributor.affiliatedAuthorHan, Song Yi-
dc.contributor.affiliatedAuthorKim, Eun Soo-
dc.contributor.affiliatedAuthorCheon, Jae Hee-
dc.citation.volume29-
dc.citation.number4-
dc.citation.startPage275-
dc.citation.endPage280-
dc.identifier.bibliographicCitationNUTRITION RESEARCH, Vol.29(4) : 275-280, 2009-
dc.identifier.rimsid37866-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers

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