Cited 40 times in
Bovine colostrum inhibits nuclear factor kappaB-mediated proinflammatory cytokine expression in intestinal epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 천재희 | - |
dc.contributor.author | 한송이 | - |
dc.contributor.author | 김원호 | - |
dc.contributor.author | 김은수 | - |
dc.contributor.author | 김태일 | - |
dc.contributor.author | 문창모 | - |
dc.contributor.author | 박재준 | - |
dc.contributor.author | 김승원 | - |
dc.date.accessioned | 2015-04-24T16:38:33Z | - |
dc.date.available | 2015-04-24T16:38:33Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0271-5317 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/103898 | - |
dc.description.abstract | Colostrum, a nutrient-rich fluid produced by female mammals immediately after giving birth, is loaded with several immune, growth, and tissue repair factors. However, it remains unknown whether bovine colostrum has anti-inflammatory effects on intestinal epithelial cells (IEC). In this study, we aimed to investigate the anti-inflammatory effects of colostrum on IEC and to elucidate its molecular mechanisms. Human colon cancer HT-29 cells were stimulated with interleukin (IL)-1beta with or without bovine colostrum. The effects of colostrum on nuclear factor kappaB (NF-kappaB) signaling in HT-29 cells were examined using real-time reverse transcriptase-polymerase chain reaction detect IL-8 and intracellar adhesion molecule-1 mRNA expression using a NF-kappaB-dependent reporter gene assay and an electrophoretic mobility shift assay. Furthermore, we assessed the expression levels of inhibitor protein of NF-kappaB-alpha, cyclooxygenase-2, and p65 proteins by Western blotting. Bovine colostrum significantly inhibited IL-1beta-induced IL-8 and intracellar adhesion molecule-1 mRNA expression. Moreover, it suppressed IL-1beta-induced NF-kappaB activation, including NF-kappaB dependent reporter gene expression in a dose-dependent manner. Finally, Western blotting revealed that colostrum decreased the cyclooxygenase-2 protein expression level, inhibited inhibitor protein of NF-kappaB-alpha degradation, and blocked translocation of p65 into the nucleus. These data demonstrated that bovine colostrum might protect against IEC inflammation by inhibiting the NF-kappaB pathway, suggesting colostrum has a therapeutic potential for intestinal inflammation | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | NUTRITION RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Active Transport, Cell Nucleus | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Anti-Inflammatory Agents/pharmacology* | - |
dc.subject.MESH | Cattle | - |
dc.subject.MESH | Cell Adhesion Molecules/antagonists & inhibitors | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | Colostrum* | - |
dc.subject.MESH | Cyclooxygenase 2/metabolism | - |
dc.subject.MESH | Cyclooxygenase 2 Inhibitors/pharmacology | - |
dc.subject.MESH | Epithelial Cells/drug effects* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Expression/drug effects | - |
dc.subject.MESH | HT29 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | I-kappa B Proteins/antagonists & inhibitors | - |
dc.subject.MESH | Inflammation/genetics | - |
dc.subject.MESH | Inflammation/metabolism* | - |
dc.subject.MESH | Interleukin-1beta/antagonists & inhibitors* | - |
dc.subject.MESH | Interleukin-8/antagonists & inhibitors* | - |
dc.subject.MESH | Intestines/cytology | - |
dc.subject.MESH | Intestines/drug effects | - |
dc.subject.MESH | NF-KappaB Inhibitor alpha | - |
dc.subject.MESH | NF-kappa B/antagonists & inhibitors* | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Transcription Factor RelA/antagonists & inhibitors | - |
dc.title | Bovine colostrum inhibits nuclear factor kappaB-mediated proinflammatory cytokine expression in intestinal epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Won Ho Kim | - |
dc.contributor.googleauthor | Min Ji An | - |
dc.contributor.googleauthor | Tae Il Kim | - |
dc.contributor.googleauthor | Eun Soo Kim | - |
dc.contributor.googleauthor | Song Yi Han | - |
dc.contributor.googleauthor | Chang Mo Moon | - |
dc.contributor.googleauthor | Jae Jun Park | - |
dc.contributor.googleauthor | Seung Won Kim | - |
dc.contributor.googleauthor | Jae Hee Cheon | - |
dc.identifier.doi | 10.1016/j.nutres.2009.03.011 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00774 | - |
dc.contributor.localId | A01079 | - |
dc.contributor.localId | A01390 | - |
dc.contributor.localId | A01636 | - |
dc.contributor.localId | A04291 | - |
dc.contributor.localId | A00804 | - |
dc.contributor.localId | A04030 | - |
dc.relation.journalcode | J02401 | - |
dc.identifier.eissn | 1879-0739 | - |
dc.identifier.pmid | 19410980 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0271531709000554 | - |
dc.subject.keyword | Bovine | - |
dc.subject.keyword | Colostrum | - |
dc.subject.keyword | NF-κB | - |
dc.subject.keyword | Intestinal | - |
dc.subject.keyword | Inflammation | - |
dc.subject.keyword | HT-29 cells | - |
dc.contributor.alternativeName | Cheon, Jae Hee | - |
dc.contributor.alternativeName | Han, Song Yi | - |
dc.contributor.alternativeName | Kim, Won Ho | - |
dc.contributor.alternativeName | Kim, Eun Soo | - |
dc.contributor.alternativeName | Kim, Tae Il | - |
dc.contributor.alternativeName | Moon, Chang Mo | - |
dc.contributor.alternativeName | Park, Jae Jun | - |
dc.contributor.affiliatedAuthor | Kim, Won Ho | - |
dc.contributor.affiliatedAuthor | Kim, Tae Il | - |
dc.contributor.affiliatedAuthor | Moon, Chang Mo | - |
dc.contributor.affiliatedAuthor | Park, Jae Jun | - |
dc.contributor.affiliatedAuthor | Han, Song Yi | - |
dc.contributor.affiliatedAuthor | Kim, Eun Soo | - |
dc.contributor.affiliatedAuthor | Cheon, Jae Hee | - |
dc.citation.volume | 29 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 275 | - |
dc.citation.endPage | 280 | - |
dc.identifier.bibliographicCitation | NUTRITION RESEARCH, Vol.29(4) : 275-280, 2009 | - |
dc.identifier.rimsid | 37866 | - |
dc.type.rims | ART | - |
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