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The third helix of the Hoxc8 homeodomain peptide enhances the efficiency of gene transfer in combination with lipofectamine.

 Jogeswar Gadi  ;  Kalyani Ruthala  ;  Kyoung-Ah Kong  ;  Hyoung Woo Park  ;  Myoung Hee Kim 
 MOLECULAR BIOTECHNOLOGY, Vol.42(1) : 41-48, 2009 
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Animals ; COS Cells ; Cell Survival/drug effects ; Cercopithecus aethiops ; DNA/metabolism ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism* ; Lipids/pharmacology* ; Mice ; Microscopy, Electron, Transmission ; Peptides/genetics ; Peptides/metabolism* ; Transfection/methods* ; Transformation, Genetic/drug effects*
Protein transduction domain ; Hoxc8 ; Homeodomain ; Oligopeptide ; Gene delivery vector
Protein transduction domains (PTDs) have been shown to cross the biological cell membranes efficiently through a receptor and energy independent mechanism. Because of its ease in membrane transducing ability, PTDs could be used as a gene delivery vector. Since we already have shown that purified Hoxc8 homeoprotein has the ability to cross the cellular membrane, we analyzed the possibility of the third helix of the Hoxc8 homeodomain as a useful gene delivery vector. For that purpose, a 16-aa long synthetic oligopeptide Hoxc8 Protein Transduction Domain (HPTD) was chemically synthesized and then tested to see whether the HPTD could form a complex with DNA or not. Gel retardation analysis revealed that the HPTD interacts with plasmid DNA efficiently but failed to transfer the DNA into the cells. However, HPTD can enhance the efficiency of gene transfer in combination with Lipofectamine which doubled the gene transfer rate into COS-7 cells compared with the DNA/Lipofectamine control. An MTT assay indicated that the amount of HPTD used in the complex for the transfection did not show any cytotoxicty in COS-7 cells. The TEM studies showed compact particle formation in the presence of HPTD. These results indicate that the HPTD could be a good candidate adjuvant molecule to enhance the gene transfer efficiency of Lipofectamine in eukaryotic cells
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1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Gadi, Jogeswar(가디죠게스)
Kong, Kyoung Ah(공경아)
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Park, Hyoung Woo(박형우) ORCID logo https://orcid.org/0000-0002-5341-9249
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