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Characterization of the major allergens of Pachycondyla chinensis in ant sting anaphylaxis patients

 E. K. Lee  ;  K. Y. Jeong  ;  D-P. Lyu  ;  Y-W. Lee  ;  J-H. Sohn  ;  K-J. Lim  ;  C-S. Hong  ;  J-W. Park 
 CLINICAL AND EXPERIMENTAL ALLERGY, Vol.39(4) : 602-607, 2009 
Journal Title
Issue Date
Adult ; Allergens/chemistry* ; Allergens/immunology* ; Allergens/isolation & purification ; Amino Acid Sequence ; Anaphylaxis/immunology* ; Animals ; Ants/immunology* ; Cloning, Molecular ; Electrophoresis, Gel, Two-Dimensional ; Female ; Humans ; Immunoglobulin E/blood ; Insect Bites and Stings/immunology* ; Male ; Middle Aged ; Molecular Sequence Data ; Sequence Homology, Amino Acid ; Spectrometry, Mass, Electrospray Ionization
ant sting anaphylaxis ; major allergen ; Pachycondyla chinensis
BACKGROUND: The ant species Pachycondyla chinensis, which has spread from Far Eastern Asia to New Zealand and North America, induces anaphylactic reactions in human with its sting. However, the major allergens of P. chinensis have not yet been characterized. METHODS: We selected seven patients with histories of anaphylaxis induced by P. chinensis. Two-dimensional electrophoresis (2-DE) was used to identify the major allergens. We subsequently performed Western blots for P. chinensis-specific IgEs, N-terminal amino acid sequencing, ESI-MS/MS, and RT-PCR using primers based on the N-terminal sequence. RESULTS: Six of the anaphylactic subjects had an IgE specific to a 23 kDa allergen of P. chinensis. Two candidates for major allergens, 23 kDa (pI 8.7) and 25 kDa (pI 6.2), were revealed by 2-DE using P. chinensis-specific IgE immunoblotting. In N-terminal sequencing and ESI-MS/MS analysis, 23 kDa (pI 8.7) and 25 kDa (pI 6.2) allergens, belonging to the protein families of antigen 5, were identified and share marked amino acid sequence similarity. The 23 kDa allergen is 206 amino acids in length and homology searches showed 54.0% and 50.0% homology with Sol i 3 and Ves v 5, respectively. CONCLUSION: The major allergens of P. chinensis are 23 kDa (pI 8.7) and 25 kDa (pI 6.2) proteins that belong to the antigen 5 family of proteins
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
Yonsei Authors
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Lee, Yong Won(이용원)
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
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