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Characterization of the major allergens of Pachycondyla chinensis in ant sting anaphylaxis patients

DC FieldValueLanguage
dc.contributor.author박중원-
dc.contributor.author이용원-
dc.contributor.author정경용-
dc.contributor.author홍천수-
dc.date.accessioned2015-04-24T16:37:11Z-
dc.date.available2015-04-24T16:37:11Z-
dc.date.issued2009-
dc.identifier.issn0954-7894-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/103855-
dc.description.abstractBACKGROUND: The ant species Pachycondyla chinensis, which has spread from Far Eastern Asia to New Zealand and North America, induces anaphylactic reactions in human with its sting. However, the major allergens of P. chinensis have not yet been characterized. METHODS: We selected seven patients with histories of anaphylaxis induced by P. chinensis. Two-dimensional electrophoresis (2-DE) was used to identify the major allergens. We subsequently performed Western blots for P. chinensis-specific IgEs, N-terminal amino acid sequencing, ESI-MS/MS, and RT-PCR using primers based on the N-terminal sequence. RESULTS: Six of the anaphylactic subjects had an IgE specific to a 23 kDa allergen of P. chinensis. Two candidates for major allergens, 23 kDa (pI 8.7) and 25 kDa (pI 6.2), were revealed by 2-DE using P. chinensis-specific IgE immunoblotting. In N-terminal sequencing and ESI-MS/MS analysis, 23 kDa (pI 8.7) and 25 kDa (pI 6.2) allergens, belonging to the protein families of antigen 5, were identified and share marked amino acid sequence similarity. The 23 kDa allergen is 206 amino acids in length and homology searches showed 54.0% and 50.0% homology with Sol i 3 and Ves v 5, respectively. CONCLUSION: The major allergens of P. chinensis are 23 kDa (pI 8.7) and 25 kDa (pI 6.2) proteins that belong to the antigen 5 family of proteins-
dc.description.statementOfResponsibilityopen-
dc.format.extent602~607-
dc.relation.isPartOfClinical and Experimental Allergy-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAllergens/chemistry*-
dc.subject.MESHAllergens/immunology*-
dc.subject.MESHAllergens/isolation & purification-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAnaphylaxis/immunology*-
dc.subject.MESHAnimals-
dc.subject.MESHAnts/immunology*-
dc.subject.MESHCloning, Molecular-
dc.subject.MESHElectrophoresis, Gel, Two-Dimensional-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHImmunoglobulin E/blood-
dc.subject.MESHInsect Bites and Stings/immunology*-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHSequence Homology, Amino Acid-
dc.subject.MESHSpectrometry, Mass, Electrospray Ionization-
dc.titleCharacterization of the major allergens of Pachycondyla chinensis in ant sting anaphylaxis patients-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorE. K. Lee-
dc.contributor.googleauthorK. Y. Jeong-
dc.contributor.googleauthorD-P. Lyu-
dc.contributor.googleauthorY-W. Lee-
dc.contributor.googleauthorJ-H. Sohn-
dc.contributor.googleauthorK-J. Lim-
dc.contributor.googleauthorC-S. Hong-
dc.contributor.googleauthorJ-W. Park-
dc.identifier.doi10.1111/j.1365-2222.2008.03181.x-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01681-
dc.contributor.localIdA02981-
dc.contributor.localIdA03572-
dc.contributor.localIdA04448-1-
dc.relation.journalcodeJ00548-
dc.identifier.pmid19178543-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2222.2008.03181.x/abstract-
dc.subject.keywordant sting anaphylaxis-
dc.subject.keywordmajor allergen-
dc.subject.keywordPachycondyla chinensis-
dc.contributor.alternativeNamePark, Jung Won-
dc.contributor.alternativeNameLee, Yong Won-
dc.contributor.alternativeNameJeong, Kyoung Yong-
dc.contributor.alternativeNameHong, Chein Soo-
dc.contributor.affiliatedAuthorPark, Jung Won-
dc.contributor.affiliatedAuthorLee, Yong Won-
dc.contributor.affiliatedAuthorJeong, Kyoung Yong-
dc.contributor.affiliatedAuthorHong, Chein Soo-
dc.citation.volume39-
dc.citation.number4-
dc.citation.startPage602-
dc.citation.endPage607-
dc.identifier.bibliographicCitationClinical and Experimental Allergy, Vol.39(4) : 602-607, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers

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