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Distinguishing between apoptosis and necrosis using a capacitance sensor

Authors
 Ri Mi Lee  ;  Hyangtae Choi  ;  Jeon-Soo Shin  ;  Kunhong Kim  ;  Kyung-Hwa Yoo 
Citation
 BIOSENSORS & BIOELECTRONICS, Vol.24(8) : 2586-2591, 2009 
Journal Title
 BIOSENSORS & BIOELECTRONICS 
ISSN
 0956-5663 
Issue Date
2009
MeSH
Animals ; Apoptosis/physiology* ; Biosensing Techniques/instrumentation* ; Electric Capacitance ; Electrochemistry/instrumentation* ; Equipment Design ; Equipment Failure Analysis ; Humans ; Mice ; Necrosis/physiopathology* ; Reproducibility of Results ; Sensitivity and Specificity
Keywords
Capacitance sensor ; Cell proliferation ; Apoptosis ; Necrosis
Abstract
Apoptosis and necrosis are two different paths for cell death. One of differences between apoptosis and necrosis is the cell morphology. Apoptotic cells shrink without loosing the integrity of their plasma membrane and break into smaller pieces called apoptotic bodies that other body cells recognize and eat. In contrast, necrotic cells swell and their plasma membrane eventually ruptures. Since the cell membrane is closely related to the capacitance (or dielectric constant), we have fabricated a capacitance sensor, which can measure the capacitance of cells, and investigated its time dependence during apoptosis and necrosis for TE2 cells induced by TNF-related apoptosis inducing ligand (TRAIL) and ethanol. The capacitance decreases monotonically during apoptosis. For necrosis, however, step-like behaviors are observed and dips are found in the dC/dt-t curves. The time-lapse images of TE2 cells, which have been taken simultaneously with the capacitance measurements, show that the dips in the dC/dt-t curves are probably due to the rupture of cell membrane. These results suggest that apoptosis and necrosis are differentiated by the capacitance measurements.
Full Text
http://www.sciencedirect.com/science/article/pii/S0956566309000360
DOI
10.1016/j.bios.2009.01.028
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kun Hong(김건홍) ORCID logo https://orcid.org/0000-0001-5639-6372
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103704
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