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Endometrial osteopontin mRNA expression and plasma osteopontin levels are increased in patients with endometriosis

Authors
 SiHyun Cho  ;  Young Sun Ahn  ;  Young Sik Choi  ;  Seok Kyo Seo  ;  Anna Nam  ;  Hye Yeon Kim  ;  Jae-Hoon Kim  ;  Ki Hyun Park  ;  Dong Jae Cho  ;  Byung Seok Lee 
Citation
 AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Vol.61(4) : 286-293, 2009 
Journal Title
 AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY 
ISSN
 1046-7408 
Issue Date
2009
MeSH
Adolescent ; Adult ; Biomarkers ; CA-125 Antigen/genetics ; CA-125 Antigen/metabolism* ; Endometriosis/diagnosis ; Endometriosis/metabolism* ; Endometriosis/pathology ; Endometriosis/physiopathology ; Endometrium/metabolism* ; Enzyme-Linked Immunosorbent Assay ; Female ; Gene Expression Profiling ; Humans ; Middle Aged ; Osteopontin/genetics ; Osteopontin/metabolism* ; RNA, Messenger/analysis ; ROC Curve
Keywords
Endometriosis ; endometrium ; mRNA ; osteopontin ; plasma
Abstract
PROBLEM: The aim of this study was to evaluate osteopontin (OPN) mRNA expression in eutopic endometrium and plasma OPN levels in patients with endometriosis. METHOD OF STUDY: A total of 79 patients with histologically confirmed endometriosis and 43 patients without endometriosis participated in this study. OPN mRNA expression in endometrial tissues was measured by real-time quantitative polymerase chain reaction (PCR) and plasma concentrations of OPN were quantified using a specific commercial sandwich enzyme-linked immunosorbent assays (ELISA). RESULTS: Osteopontin mRNA expression in endometrial tissue was significantly higher in women with endometriosis than in controls (P = 0.010). The mean plasma levels of OPN (mean +/- S.E.M.) in patients with endometriosis and controls were 407.31 +/- 37.80 ng/mL and 165.84 +/- 19.29 ng/mL, respectively (P < 0.001). Receiver operating characteristic (ROC) analysis for plasma OPN revealed an area under the curve (AUC) of 0.894, with a sensitivity of 93.0%, specificity of 72.4%, positive likelihood ratio of 3.37, and negative likelihood ratio of 0.1 using a cut-off value of 167.68 ng/mL. CONCLUSION: Osteopontin may be involved in the pathogenesis of endometriosis and plasma OPN may be a useful non-invasive marker for the diagnosis of endometriosis.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0897.2009.00692.x/abstract
DOI
10.1111/j.1600-0897.2009.00692.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jae Hoon(김재훈) ORCID logo https://orcid.org/0000-0001-6599-7065
Kim, Hye Yeon(김혜연)
Nam, An Na(남안나)
Park, Ki Hyun(박기현)
Seo, Seok Kyo(서석교) ORCID logo https://orcid.org/0000-0003-3404-0484
Lee, Byung Seok(이병석) ORCID logo https://orcid.org/0000-0001-6001-2079
Cho, Dong Je(조동제)
Cho, Si Hyun(조시현) ORCID logo https://orcid.org/0000-0003-2718-6645
Choi, Young Sik(최영식) ORCID logo https://orcid.org/0000-0002-1157-4822
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103501
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