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Eukaryotic translation initiator protein 1A isoform, CCS-3, enhances the transcriptional repression of p21CIP1 by proto-oncogene FBI-1 (Pokemon/ZBTB7A).

Authors
 Won-Il Choi  ;  Youngsoo Kim  ;  Yuri Kim  ;  Mi-young Yu  ;  Jungeun Park  ;  Choong-Eun Lee  ;  Bu-Nam Jeon  ;  Dong-In Koh  ;  Man-Wook Hur 
Citation
 CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, Vol.23(4-6) : 359-370, 2009 
Journal Title
 CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 
ISSN
 1015-8987 
Issue Date
2009
MeSH
Amino Acid Sequence ; Cell Differentiation ; Cyclin-Dependent Kinase Inhibitor p21/genetics ; Cyclin-Dependent Kinase Inhibitor p21/metabolism* ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism* ; Humans ; Immunoprecipitation ; Molecular Sequence Data ; Peptide Elongation Factor 1/genetics ; Peptide Elongation Factor 1/metabolism* ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Protein Structure, Tertiary ; Transcription Factors/genetics ; Transcription Factors/metabolism* ; Transcription, Genetic
Keywords
eEF1A ; CCS-3 ; Transcription ; Repression ; FBI-1 ; p21 ; Pokemon ; POZ domain ; Co-repressors
Abstract
FBI-1, a member of the POK (POZ and Kruppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis. eEF1A is a eukaryotic translation elongation factor involved in several cellular processes including embryogenesis, oncogenic transformation, cell proliferation, and cytoskeletal organization. CCS-3, a potential cervical cancer suppressor, is an isoform of eEF1A. We found that eEF1A forms a complex with FBI-1 by co-immunoprecipitation, SDS-PAGE, and MALDI-TOF Mass analysis of the immunoprecipitate. GST fusion protein pull-downs showed that FBI-1 directly interacts with eEF1A and CCS-3 via the zinc finger and POZ-domain of FBI-1. FBI-1 co-localizes with either eEF1A or CCS-3 at the nuclear periplasm. CCS-3 enhances transcriptional repression of the p21CIP1 gene (hereafter referred to as p21) by FBI-1. The POZ-domain of FBI-1 interacts with the co-repressors, SMRT and BCoR. We found that CCS-3 also interacts with the co-repressors independently. The molecular interaction between the co-repressors and CCS-3 at the POZ-domain of FBI-1 appears to enhance FBI-1 mediated transcriptional repression. Our data suggest that CCS-3 may be important in cell differentiation, tumorigenesis, and oncogenesis by interacting with the proto-oncogene FBI-1 and transcriptional co-repressors
Full Text
http://www.karger.com/Article/Abstract/218182
DOI
10.1159/000218182
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yu Ri(김유리)
Yu, Mi Young(유미영)
Jeon, Bu Nam(전부남)
Choi, Won Il(최원일)
Hur, Man Wook(허만욱) ORCID logo https://orcid.org/0000-0002-3416-1334
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103484
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