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Eukaryotic translation initiator protein 1A isoform, CCS-3, enhances the transcriptional repression of p21CIP1 by proto-oncogene FBI-1 (Pokemon/ZBTB7A).

DC Field Value Language
dc.contributor.author김유리-
dc.contributor.author유미영-
dc.contributor.author전부남-
dc.contributor.author최원일-
dc.contributor.author허만욱-
dc.date.accessioned2015-04-24T16:25:22Z-
dc.date.available2015-04-24T16:25:22Z-
dc.date.issued2009-
dc.identifier.issn1015-8987-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/103484-
dc.description.abstractFBI-1, a member of the POK (POZ and Kruppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis. eEF1A is a eukaryotic translation elongation factor involved in several cellular processes including embryogenesis, oncogenic transformation, cell proliferation, and cytoskeletal organization. CCS-3, a potential cervical cancer suppressor, is an isoform of eEF1A. We found that eEF1A forms a complex with FBI-1 by co-immunoprecipitation, SDS-PAGE, and MALDI-TOF Mass analysis of the immunoprecipitate. GST fusion protein pull-downs showed that FBI-1 directly interacts with eEF1A and CCS-3 via the zinc finger and POZ-domain of FBI-1. FBI-1 co-localizes with either eEF1A or CCS-3 at the nuclear periplasm. CCS-3 enhances transcriptional repression of the p21CIP1 gene (hereafter referred to as p21) by FBI-1. The POZ-domain of FBI-1 interacts with the co-repressors, SMRT and BCoR. We found that CCS-3 also interacts with the co-repressors independently. The molecular interaction between the co-repressors and CCS-3 at the POZ-domain of FBI-1 appears to enhance FBI-1 mediated transcriptional repression. Our data suggest that CCS-3 may be important in cell differentiation, tumorigenesis, and oncogenesis by interacting with the proto-oncogene FBI-1 and transcriptional co-repressors-
dc.description.statementOfResponsibilityopen-
dc.format.extent359~370-
dc.relation.isPartOfCELLULAR PHYSIOLOGY AND BIOCHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCyclin-Dependent Kinase Inhibitor p21/genetics-
dc.subject.MESHCyclin-Dependent Kinase Inhibitor p21/metabolism*-
dc.subject.MESHDNA-Binding Proteins/genetics-
dc.subject.MESHDNA-Binding Proteins/metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHImmunoprecipitation-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPeptide Elongation Factor 1/genetics-
dc.subject.MESHPeptide Elongation Factor 1/metabolism*-
dc.subject.MESHProtein Isoforms/genetics-
dc.subject.MESHProtein Isoforms/metabolism-
dc.subject.MESHProtein Structure, Tertiary-
dc.subject.MESHTranscription Factors/genetics-
dc.subject.MESHTranscription Factors/metabolism*-
dc.subject.MESHTranscription, Genetic-
dc.titleEukaryotic translation initiator protein 1A isoform, CCS-3, enhances the transcriptional repression of p21CIP1 by proto-oncogene FBI-1 (Pokemon/ZBTB7A).-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorWon-Il Choi-
dc.contributor.googleauthorYoungsoo Kim-
dc.contributor.googleauthorYuri Kim-
dc.contributor.googleauthorMi-young Yu-
dc.contributor.googleauthorJungeun Park-
dc.contributor.googleauthorChoong-Eun Lee-
dc.contributor.googleauthorBu-Nam Jeon-
dc.contributor.googleauthorDong-In Koh-
dc.contributor.googleauthorMan-Wook Hur-
dc.identifier.doi10.1159/000218182-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02465-
dc.contributor.localIdA03517-
dc.contributor.localIdA04126-
dc.contributor.localIdA04350-
dc.contributor.localIdA00778-
dc.relation.journalcodeJ00501-
dc.identifier.eissn1421-9778-
dc.identifier.pmid19471103-
dc.identifier.urlhttp://www.karger.com/Article/Abstract/218182-
dc.subject.keywordeEF1A-
dc.subject.keywordCCS-3-
dc.subject.keywordTranscription-
dc.subject.keywordRepression-
dc.subject.keywordFBI-1-
dc.subject.keywordp21-
dc.subject.keywordPokemon-
dc.subject.keywordPOZ domain-
dc.subject.keywordCo-repressors-
dc.contributor.alternativeNameKim, Yu Ri-
dc.contributor.alternativeNameYu, Mi Young-
dc.contributor.alternativeNameJeon, Bu Nam-
dc.contributor.alternativeNameChoi, Won Il-
dc.contributor.alternativeNameHur, Man Wook-
dc.contributor.affiliatedAuthorYu, Mi Young-
dc.contributor.affiliatedAuthorJeon, Bu Nam-
dc.contributor.affiliatedAuthorChoi, Won Il-
dc.contributor.affiliatedAuthorHur, Man Wook-
dc.contributor.affiliatedAuthorKim, Yu Ri-
dc.citation.volume23-
dc.citation.number4-6-
dc.citation.startPage359-
dc.citation.endPage370-
dc.identifier.bibliographicCitationCELLULAR PHYSIOLOGY AND BIOCHEMISTRY, Vol.23(4-6) : 359-370, 2009-
dc.identifier.rimsid36032-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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