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Identification of proteins indicating radiation-induced hepatic toxicity in cirrhotic rats

 Sook In CHUNG  ;  Jinsil SEONG  ;  Young Nyun PARK  ;  Won Woo KIM  ;  Hae Jin OH  ;  Kwang-Hyub HAN 
 JOURNAL OF RADIATION RESEARCH, Vol.51(6) : 643-650, 2010 
Journal Title
Issue Date
Animals ; Biomarkers/metabolism ; Enzyme Precursors/metabolism ; Glucuronidase/metabolism ; Hepatocyte Growth Factor/metabolism ; Liver/injuries ; Liver/metabolism ; Liver/radiation effects ; Liver Cirrhosis, Experimental/complications* ; Liver Cirrhosis, Experimental/metabolism* ; Liver Cirrhosis, Experimental/pathology ; Male ; Proteins/metabolism* ; Proteomics ; Radiation Injuries, Experimental/complications* ; Radiation Injuries, Experimental/metabolism* ; Radiation Injuries, Experimental/pathology ; Radiation Tolerance ; Rats ; Rats, Wistar
Liver cirrhosis ; Radiation ; Proteomics ; Hepatic injury
Radiation therapy (RT) has been emerging as one of the palliative treatments for locally advanced hepatocellular carcinoma (HCC). However, hepatic toxicity is a major obstacle in radiotherapy for HCC. The purpose of this study is to identify proteins indicating radiation-induced hepatic toxicity in cirrhotic rats, which can be used as possible biomarkers. Liver cirrhosis was induced in Wistar rats with thioacetamide (TAA) 0.3 g/L in drinking water for 9 weeks. The development of liver cirrhosis was observed histologically. Radiation hepatic injury was induced by treating 1/3 of the liver with 10 Gy single dose radiation. To find out commonly expressed proteins, liver tissue and serum were analyzed using two-dimensional electrophoresis and quadrupole time of flight mass spectrometry. Identified proteins were validated using western blotting. Histological examination showed that the degree of hepatic fibrosis increased by radiation in liver cirrhosis. It was associated with a decrease in the proliferation of cell nuclear antigen and an increase of apoptosis. The proteomic analysis of liver tissue and serum identified 60 proteins which showed significant change in expression between the TAA-alone and TAA-plus-radiation groups. Among these, an increase of heparanase precursor and decrease of hepatocyte growth factor were shown commonly in liver tissue and serum following radiation. Hepatic fibrosis increased following radiation in cirrhotic rats. These proteins might be useful in detecting and monitoring radiation-induced hepatic injury.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Kim, Won Woo(김원우)
Park, Young Nyun(박영년) ORCID logo https://orcid.org/0000-0003-0357-7967
Seong, Jin Sil(성진실) ORCID logo https://orcid.org/0000-0003-1794-5951
Oh, Hae Jin(오해진)
Chung, Sook In(정숙인) ORCID logo https://orcid.org/0000-0002-7915-9203
Han, Kwang-Hyub(한광협) ORCID logo https://orcid.org/0000-0003-3960-6539
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