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The potential effects of ethyl acetate fraction from Curcuma longa L. on lipolysis in differentiated 3T3-L1 adipocytes

 Jeongmin Lee  ;  Ho-Geun Yoon  ;  Yoo-Hyun Lee  ;  Jeongjin Park  ;  Yanghee You  ;  Kyungmi Kim  ;  Ja-Young Jang  ;  Ji-Won Yang  ;  Woojin Jun 
 JOURNAL OF MEDICINAL FOOD, Vol.13(2) : 364-370, 2010 
Journal Title
Issue Date
3T3-L1 Cells ; Adipocytes/drug effects* ; Adipocytes/metabolism ; Animals ; Biological Transport ; Curcuma/chemistry* ; Dose-Response Relationship, Drug ; Glucose/metabolism* ; Glucose Transporter Type 4/metabolism* ; Glycerol/metabolism ; Lipase/genetics ; Lipase/metabolism ; Lipolysis/drug effects* ; Mice ; Organelles/drug effects ; Organelles/metabolism ; Plant Extracts/pharmacology* ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sterol Esterase/genetics ; Sterol Esterase/metabolism ; Triglycerides/metabolism
The effects of the turmeric ethyl acetate fraction (TEF) from the methanolic extract from Curcuma longa L. on lipid metabolism and underlying mechanisms of lipolysis were investigated in 3T3-L1 adipocytes. The intracellular lipid droplets were stained with Oil red O dye and quantified. Compared to the control, lipid accumulation was significantly decreased by 46.6% with treatment by TEF at the concentration of 20 microg/mL. The intracellular triglyceride (TG) level was also reduced by 37.9% at the concentration of 20 microg/mL. To determine the mechanism for TG content reduction, levels of glucose uptake and glycerol release were measured. Incubation of the 3T3-L1 adipocytes with TEF for 4 hours significantly lowered the cellular level of glucose in a dose-dependent manner. Furthermore, cellular expression of insulin-responsive glucose transporter (GLUT)-4 was decreased by 46%, indicating that reduced glucose uptake was due to a decrease in cellular GLUT-4 expression. In addition, the level of free glycerol released into the cultured medium was increased by 36.4% with the treatment by TEF. In subsequent measurements using quantitative real-time polymerase chain reaction, mRNA levels of hormone-sensitive lipase (HSL) and adipose TG lipase (ATGL) were elevated by 34.8% and 16.9%, respectively, at the concentration of 20 microg/mL. These results suggest that TEF partially inhibits lipogenesis by the suppression of glucose uptake via the decreased expression of cellular GLUT-4 and stimulates lipolysis through the induction of HSL and/or ATGL gene expression, resulting in the increased glycerol release.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Ho Geun(윤호근) ORCID logo https://orcid.org/0000-0003-2718-3372
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