0 222

Cited 11 times in

The potential effects of ethyl acetate fraction from Curcuma longa L. on lipolysis in differentiated 3T3-L1 adipocytes

Authors
 Jeongmin Lee  ;  Ho-Geun Yoon  ;  Yoo-Hyun Lee  ;  Jeongjin Park  ;  Yanghee You  ;  Kyungmi Kim  ;  Ja-Young Jang  ;  Ji-Won Yang  ;  Woojin Jun 
Citation
 JOURNAL OF MEDICINAL FOOD, Vol.13(2) : 364-370, 2010 
Journal Title
 JOURNAL OF MEDICINAL FOOD 
ISSN
 1096-620X 
Issue Date
2010
MeSH
3T3-L1 Cells ; Adipocytes/drug effects* ; Adipocytes/metabolism ; Animals ; Biological Transport ; Curcuma/chemistry* ; Dose-Response Relationship, Drug ; Glucose/metabolism* ; Glucose Transporter Type 4/metabolism* ; Glycerol/metabolism ; Lipase/genetics ; Lipase/metabolism ; Lipolysis/drug effects* ; Mice ; Organelles/drug effects ; Organelles/metabolism ; Plant Extracts/pharmacology* ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sterol Esterase/genetics ; Sterol Esterase/metabolism ; Triglycerides/metabolism
Abstract
The effects of the turmeric ethyl acetate fraction (TEF) from the methanolic extract from Curcuma longa L. on lipid metabolism and underlying mechanisms of lipolysis were investigated in 3T3-L1 adipocytes. The intracellular lipid droplets were stained with Oil red O dye and quantified. Compared to the control, lipid accumulation was significantly decreased by 46.6% with treatment by TEF at the concentration of 20 microg/mL. The intracellular triglyceride (TG) level was also reduced by 37.9% at the concentration of 20 microg/mL. To determine the mechanism for TG content reduction, levels of glucose uptake and glycerol release were measured. Incubation of the 3T3-L1 adipocytes with TEF for 4 hours significantly lowered the cellular level of glucose in a dose-dependent manner. Furthermore, cellular expression of insulin-responsive glucose transporter (GLUT)-4 was decreased by 46%, indicating that reduced glucose uptake was due to a decrease in cellular GLUT-4 expression. In addition, the level of free glycerol released into the cultured medium was increased by 36.4% with the treatment by TEF. In subsequent measurements using quantitative real-time polymerase chain reaction, mRNA levels of hormone-sensitive lipase (HSL) and adipose TG lipase (ATGL) were elevated by 34.8% and 16.9%, respectively, at the concentration of 20 microg/mL. These results suggest that TEF partially inhibits lipogenesis by the suppression of glucose uptake via the decreased expression of cellular GLUT-4 and stimulates lipolysis through the induction of HSL and/or ATGL gene expression, resulting in the increased glycerol release.
Full Text
http://online.liebertpub.com/doi/abs/10.1089/jmf.2009.1276
DOI
10.1089/jmf.2009.1276
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Ho Geun(윤호근) ORCID logo https://orcid.org/0000-0003-2718-3372
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102634
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse